Structure of PDB 7k58 Chain A

Receptor sequence

>7k58A (length=4443) Species: 5911 (Tetrahymena thermophila)

KYSKRIAWMKTTICDSLQLKDMIVEESFQYEKNKNLLEQFLSGEGLNKIF
AYYQVQEQAQNTTGDLEKIQDKAVWFLRITNPADDKKKASQQDGNDNDII
FGEITPNTVPMLNALMESVYSRQIDHIITEKIQFAEEEQVLEFQQHSNKF
SSEVREAINLLDYEAISGLSESEKMQHYEMKFNEWINLISSQLRKDEKDA
GPATELIYWRSRMQKITNWSEQLKSKDFQIVKASLQRHKNHDNQRPRGDE
SKLNEAKDNVKYLTTLEKFIPLYNGTPQQIIDTLPALMNAIKMIHTTTDK
MTGLFIKITNQMIKNCKDRILNKMIWEQDPAELIEVLGSCIKLYCEYKKC
YNDTKEKVADMPKGKTSDAQIFGKFDTFVRRLQKLIEIFSNIQQFNALLE
GMDVLTNKFKKIIDDFKKKGHNLLDTANNKFDRDWVEFNVEISHLDGELQ
NFIDNNFNRFREYSLKLLHKFQSTIKRHNLTSRYNAILHNYATELDTIQR
VFQDQKSNPPLVRNMPPEAGKIIWARHLFQKITGPINTEIRRYYGSYNTL
GKQLTIYEMWFYQDWVNKIEQSKAALQATLIVRHDENKKLYVNFDLEIMQ
LIREAKCLDRQGIEIPESARIILLQEDKFKTYYNELLYALKEYERINSKI
KPICKNLLLPHIEDLDLKLRPGMVTLTWTSMNIESYLYYVHQGLKKLEQL
IINVNDIIENRIENNLKTVSKVVLVHLPLDSFVQLQEEYINSKTDFLTSK
NVEVERAVDDLLQTIMLYPLDPHVDPVLPEETKRIKRYYFWYFYQALLNS
TQNSLNAMKYRFFQVEVQLNGDKVTLIQKSINRAATAVLRCSKHLYNWDQ
ATFYDMIACDKEIVKVILLLTGSIQGTKNKVNEFLSGFTKFEWLCKESIQ
ESIKKFSKNGPTLQNYEDQLKKFSQIEEEIEKIVPTYKIGAMELMTHNIC
TSLSTWAKEWKLQYSQDLHKRARQLLDSLTEQTKMLSTKLSKPVKDIDSL
GYVMETLEQIRKEQAEIDMKFNPVQEMYSLLDNYLPGGITDKDEMDARSL
LRRNWDILIQQAEIKGKEYQHKQAIYLKELKQSIKDFTNQVSIFRRDYEK
NGPMERLRRFEDEYDVKYQMYKINARGENLFGLQNQKYPELEKTDAEIKN
LNKLYNLYDSVIKNIQQFKEKSWQDVSLAKMEEDAGKYGEQCSRLPKDLK
EWQAYRDLKNYIDSLREQLPLIIPRHWEKIKEITNTKLNYENPDQFYIEE
IMGALDFREDIEDITESADKQLKIRTGLDEINLYWNDMQFQFGIWGKRML
NGLIVGTILERLEEDQLQLSTFNSQRHVTPFKAEVENLIRTFSDVNDTLD
MWVKVQKLWTSLEPVFTGGDIARQMPLQAKQFQGIDKNWMKIMEKAVETK
KVIPCCQNDMLKDFLPDLNRKLEDCQKMLEAYLEGKRKKFPRFYFVSNPT
LLKILSQGSEPTSIQEDFEKLFDAITKVTFESAKDKKNPALKQITQIQQV
IGRNEENISLTGYYVKCEGNIEDWLKKLEQNMQQTLKDIASAAAQQVFQV
GLKEFVSSQASQIALLGLQILWTSKVNEGLERLSRNERNAMDIKRNEIKE
HMNILSSMCLEDLNGAVERTKVETLVTIQVHQKDISMDLKCKDVNDFEWQ
KQTRIAWKTDIDECIISITDWDSPYSYEFLGAKERLCITPLTDRCYITLA
QAMSMYYGGAPAGPAGTGKTETVKDLGRTLGVFVVVTNCSDQHRYRDMAK
IFKGLVQSGLWGCFDEFNRIDLEVLSVVAMQVESITTARKQHMKKFMFPE
EEIEIELIPTVSYFITMNPGYAGRQELPENLKVLFRGVSMMVPDREIIIK
VKLASVGYLQIDLLAKKFNVLYRLCEEQLSKQRHYDFGLRNILSVLRTAG
NTKRQEIKSDEEMLLMRSLRDMNLSKLVADDIPLFNGLLADIFPKLKEVP
KKLYPDVEKKIPEEINAESYLINTPSFQLKIIQLYETCLVRHGFMLVGPT
GSGKSTIMKILTEVLTKLGSPHKIVIMNPKAITAEQMYGVKSEISDDWIP
GVFSTIWAKSNNRALKHTTWITCDGPVDAIWIENLNTVLDDNKILTLANG
ERIAMTENCKVVFEVENLNNASPATVSRCGQVYVSPTDLGYEAVIEGWIR
NRKASGRAEESDKLGNILRKYLINMRFIELQSKECKEPMMDTSPVISVIN
ILNLLTGCLQYFVQTQRTLSEQEYEKFIVYSMAWAIGGIYEAQDRVRFHE
LLLAKNAPIPQKGKENETVFDYYVSQDYLDWKICSPEEWVPPQSLQFSQL
LLPTLDSFRAEMLLNFILTQPKSHTCSNSALLIGGSGTAKTSSVLLYCNK
FDPQKMLFKRTNFSSATSPFMFQSTIEAECDFKVGKEFAPPGNKMMTIFI
DDMSMPFVNKWGDQITLELVRQLIETGGFYMLDKTQRGNQRKMKNLQYIG
AMNHPGGGRNDIPNRLKRQFFIFNMILPLSIEGIYGPIIKHMFKQKYFSD
STYKVIESLTSATIALWNKVKSTMLPTPAKFHYVFNMRELSRIFKGILTC
KKDTINDAPKSMKIKPELFLVGLWRHEAERVLADKLVNNKDKDTVMGYIQ
EVSLESFSQIENEILEKYSSEKTFLFCDFLRPDVINEDGIIEEEAPKIYE
AIDSLTELRKRCNFLLSFYNDRNPSKKMPLVLFDDALKHLLRISRIIRQP
RSSGLLVGVGGSGKQSLTRLAGFIGKNLIQQIIVTKTYSDKDLKEDIKKG
FDDAGHLGKQVTFLMTDSEVKKEEFLEYINMVLSTGEIPNLLAKDEREVW
LGDISQAYCKEKNLGNIDPPQSELWTYFVDRVRDNFHIMLCFSPVGQKFR
ERARKFPALFNECTIDWFLPWPEEALVSVAETFIKNFDKLDTKEETKQEL
MKHMGNVHLMVNEICDEYYQKMRRQVYVTPKSFLSYLNSYKTLYIEKYDE
LDQQEESFKIGLNKIQEATITINQMEISLKEEEIQLNEATEKTNQLLANL
DKESKKANQKGEEVAATNKQCEIQAEQISKEKEEAERELEAALPALRRAQ
EAVDSIESKDIVELKANKKPLDIIKYIMDAVLVFFKARLIPIQIEERVFN
KKEGKAVLFLKESYDESGIQTLGDMNFMKKLKEFEKDSINEETIELLEPY
LNQSEDWFNDTFATKASKAAAGILKWAFAIYEYHQKSKIVKPKRIQVAIA
EGRQAIALKELEKAREDLAQIQAYIKNLKDVYTKQMEEKNELEMKAAKTK
KKINTARTLITSLSGEKDRWGKGAQDISDQKRKLVGNVSLSTAFISYCGP
FNAEYRNKLAQQRFVVDMKKRGVPVTPGLELTSFLIDDATIGEWNLQGLP
KDDLSIQNGIMVTNSARYPLFIDPQGQGQNWIRNKLSASIIPERCITTLS
HPKFKDMFLKYCMESGLTLIVENIENEVDPMMDPVLERQIIVKGKTQFVN
VAGTEMELSKEFKLFMTCRLANPSFSPELSAKTTIIDFTVTQSGLEQQLL
GKVISKEQKALEDSLNQLLADVNQNQKDLQRLDKNLLERLINSQGNLLDD
TELMDVLNNTKTQAKEVAAKLIDAEIKTKEINEKREQYRPVAIRGSAIYF
TMIEVSLVNWMYNSSLEQFLKLFIESIDLSEKAQLPSNRVKNIISFLTFH
VYRYVNRGLFEKDKITFILMMAFKILTTAGTISSGDVSLFLKSGDALDIK
SERQKQISYLEDNQWLNILALSKHTFSGQTLPFFKELPDLISRSENQWRN
WIDKNDPENFPIPDFAESINQEKEIGSFISLCLVRSLRNDRTLIATQNFI
SNVLGKEFTDPISYPIEGIWQESSNMDPVLFLLSAGADPTSSIDELAKKK
KKFPCEKVSMGEGQERVARQVIMKGFVEGGWVILQNCHLGLKFMEEIETL
VSPINQIHEDFRLWITCEQHPKFPLGLLQKTLKVTNEPPKGLKAGLYKTF
TTIITQEFIDKVDHSNWRSLIFTICFLHSIVIERKKFGPLGWCVPYEYNY
SDLEASLLYIEKYLTNLMSTPHNLPISMNVVRYMICEVQYGGRITDDLDR
ELFITYGETYLKDGIFGNDYFFYDIMVDQKFKYRIPQNPSAELIKYQEYI
AKVPTVDNPEVFGLHSNADLTFRLKESKEMINTVMETRPKDSSVGGGKTR
EEIVQDKAKDMLKNLPPDYNDVEVRELVSKLGGPNPKTSTERGMTVPLNI
FLYQEVTRMQRVIGLVRKTLQDTILAIDGQIIMTPEILEAINAIYDAKVP
NSWLYDPSGAEISWLLPNLGSWSTSLSDRNKQLNDWLRSGQRPILFWLTG
FFNPQGFLTGMKQEVTRNHKGKGGEAWSLDDVVYSTTVKEREKEKDIEQP
PAEGVYIKGLYLEGCKWSKNGLDDSDPKKIFADLPILHVSAINKKMSNTY
LCPVYKYPKRTDKYLIFRVGLPCEGSNNPSHWKLRGVALLCST

3D structure

• PDB: 7k58 Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism.
• Chain: A
• Resolution: 4.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	L2460 L2461 T2463 G2496 T2497 A2498 K2499 T2500 S2501 M2696 R2697	L2301 L2302 T2304 G2337 T2338 A2339 K2340 T2341 S2342 M2537 R2538
BS02	ATP	A	Y2129 L2130 I2131 F2136 T2159 G2160 G2162 K2163 S2164 T2165 E2273 L2298 H2483 R2624 R2627	Y1970 L1971 I1972 F1977 T2000 G2001 G2003 K2004 S2005 T2006 E2114 L2139 H2324 R2465 R2468
BS03	ADP	A	L2839 V2840 F2842 G2870 S2871 G2872 K2873 Q2874 S2875 W3030	L2680 V2681 F2683 G2711 S2712 G2713 K2714 Q2715 S2716 W2871
BS04	MG	A	S2164 E2273	S2005 E2114

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005858 axonemal dynein complex
• GO:0005874 microtubule
• GO:0005929 cilium
• GO:0030286 dynein complex

External links

• PDB: RCSB:7k58, PDBe:7k58, PDBj:7k58
• PDBsum: 7k58
• PubMed: 34556869
• UniProt: Q22A67