Structure of PDB 7gep Chain A

Receptor sequence

>7gepA (length=469) Species: 37762 (Escherichia coli B) [Search protein sequence]

LLRCRLPGGVITTKQWQAIDKFAGENTIYGSIRLTNRQTFQFHGILKKNV
KPVHQMLHSVGLDALNDMNRNVLCTSNPYESQLHAEAYEWAKKISEHLLP
RTEPILGQTYLPRKFKTTVVIPPQNDIDLHANDMNFVAIAENGKLVGFNL
LVGGGLSIEHGNKKTYARTASEFGYLPLEHTLAVAEAVVTTQRDWGNRTD
RKNAKTKYTLERVGVETFKAEVERRAGIKFEPIRPYEFTGRGDRIGWVKG
IDDNWHLTLFIENGRILDYPARPLKTGLLEIAKIHKGDFRITANQNLIIA
GVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERFL
PSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRY
NLHLGGNRIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDF
TVRAGIIRPVLDPARDLWD

3D structure

PDB

7gep Probing the catalytic mechanism of sulfite reductase by X-ray crystallography: structures of the Escherichia coli hemoprotein in complex with substrates, inhibitors, intermediates, and products.

Chain

Resolution

2.4 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R83 R153 K215 K217 A394 C434 C440 C479 C483
Catalytic site (residue number reindexed from 1)	R3 R70 K114 K116 A293 C333 C339 C378 C382
Enzyme Commision number	1.8.1.2: assimilatory sulfite reductase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	C434 S436 C440 A443 G478 C479 C483	C333 S335 C339 A342 G377 C378 C382
BS02	SRM	A	L81 R83 R113 T115 N116 R117 H123 R214 K215 K217 L257 S258 Q396 C434 V435 T439 C440 N481 G482 C483 R485	L1 R3 R33 T35 N36 R37 H43 R113 K114 K116 L156 S157 Q295 C333 V334 T338 C339 N380 G381 C382 R384

Gene Ontology

	Molecular Function
GO:0004783	sulfite reductase (NADPH) activity
GO:0016491	oxidoreductase activity
GO:0020037	heme binding
GO:0050661	NADP binding
GO:0051536	iron-sulfur cluster binding
GO:0051539	4 iron, 4 sulfur cluster binding

	Biological Process
GO:0008652	amino acid biosynthetic process

	Cellular Component
GO:0009337	sulfite reductase complex (NADPH)

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:7gep, PDBe:7gep, PDBj:7gep
PDBsum	7gep
PubMed	9315849
UniProt	P17846\|CYSI_ECOLI Sulfite reductase [NADPH] hemoprotein beta-component (Gene Name=cysI)

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