Structure of PDB 7fry Chain A

Receptor sequence

>7fryA (length=422) Species: 9606 (Homo sapiens)

AFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVER
LKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAI
ALDTKGPGSGGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALG
PEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLA
QKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCI
MLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPT
EVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQA
ARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLV
IVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7fry Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: A
• Resolution: 1.958 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	A	L443 T444 T445 T446 S449 W494 R501 G526 R528 G530 S531 G532 Y533 T534	L322 T323 T324 T325 S328 W373 R380 G405 R407 G409 S410 G411 Y412 T413
BS02	OXL	A	K282 E284 A305 G307 D308 T340	K161 E163 A184 G186 D187 T219
BS03	MG	A	E284 D308	E163 D187
BS04	Y0T	A	F38 L39 Y402 Q405 L406 E409	F16 L17 Y281 Q284 L285 E288

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7fry, PDBe:7fry, PDBj:7fry
• PDBsum: 7fry
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)