Structure of PDB 7eld Chain A

Receptor sequence

>7eldA (length=1137) Species: 3702 (Arabidopsis thaliana)

RRYQLDVLEQAKAKNTIAFLETGAGKTLIAILLIKSVHKDLMSQNRKMLS
VFLVPKVPLVYQQAEVIRNQTCFQVGHYCGEMGQDFWDSRRWQREFESKQ
VLVMTAQILLNILRHSIIRMETIDLLILDECHHAVKKHPYSLVMSEFYHT
TPKDKRPAIFGMTASPVNLKGVSSQVDCAIKIRNLETKLDSTVCTIKDRK
ELEKHVPMPSEIVVEYDKAATMWSLHETIKQMIAAVEEAAQASSGAANLI
HKLRAINYTLAELGQWCAYKVGQSFLSALQSDERVNFQVDVKFQESYLSE
VVSLLQCELLEGASLIKLLLKYQHTADFRAIVFVERVVAALVLPKVFAEL
PSLSFIRCASMIGHNNSQEMKSSQMQDTISKFRDGHVTLLVATSVAEEGL
DIRQCNVVMRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNVSHAAFLR
NARNSEETLRKEAIERTDLSHLKDTSRLISIDAVPGTVYKVEATGAMVSL
NSAVGLVHFYCSQLPGDRYAILRPEFSMEKHEKPGGHTEYSCRLQLPCNA
PFEILEGPVCSSMRLAQQAVCLAACKKLHEMGAEVCESSKLFHSEFAILF
GNELDAEVLSMSMDLYVARAMITKASLAFKGSLDITENQLSSLKKFHVRL
MSIVLDYLFVPVTGINWELVEKITKTLMMADGCMVAEDLIGKRFYVDSIC
YDMSAETSFPRKEGYLGPLEYNTYADYYKQKYGVDLNCKQQPLIKGRGVS
YVLDKTYYVFLPPELCVVHPLSGSLIRGAQRLPSIMRRVESMLLAVQLKN
LISYPIPTSKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYP
QKHEGQLTRMRQQMVSNMVLYQFALVKGLQSYRVLSSKTLADVVEALIGV
YYVEGGKIAANHLMKWIGIDFVGLERALKYEFKEKGLLVEAITHASRPSS
GVSCYQRLEFVGDAVLDHLITRHLFFTYTSLPPGRLTDLRAAAVNNENFA
RVAVKHKLHLYLRHGSSALEKQIREFVKEVQKVLGDIVESIAGAIFLDSG
KDTTAAWKVFQPLLQPMVTPETLPMHPVRELQERCQQQAEGLEYKASRSG
NTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAAL

3D structure

• PDB: 7eld Structural basis of microRNA processing by Dicer-like 1.
• Chain: A
• Resolution: 4.6 Å

Enzymatic activity

• Enzyme Commision number: 3.1.26.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P304 K305 E330 M331 G332 D334 W336 T354 Q356 I357 V384 K386 H387 K419 S423 H532 R535 R673 V674 S697 M698 I699 H701 V732 K752 S849 Y856 R901 R1242 K1243 Y1258 K1262 V1300 D1302 K1303 Y1306 V1307 D1626 M1732 H1733 R1736 Q1739 E1740 Q1743 Q1744 S1754 K1779 K1780	P55 K56 E81 M82 G83 D85 W87 T105 Q107 I108 V135 K137 H138 K170 S174 H251 R254 R336 V337 S360 M361 I362 H364 V395 K415 S512 Y519 R564 R711 K712 Y727 K731 V752 D754 K755 Y758 V759 D988 M1075 H1076 R1079 Q1082 E1083 Q1086 Q1087 S1097 K1122 K1123

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0003725 double-stranded RNA binding
• GO:0004386 helicase activity
• GO:0004519 endonuclease activity
• GO:0004525 ribonuclease III activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016891 RNA endonuclease activity, producing 5'-phosphomonoesters
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing
• GO:0010267 ta-siRNA processing
• GO:0030422 siRNA processing
• GO:0031047 regulatory ncRNA-mediated gene silencing

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm

External links

• PDB: RCSB:7eld, PDBe:7eld, PDBj:7eld
• PDBsum: 7eld
• PubMed: 34593993
• UniProt: Q9SP32|DCL1_ARATH Endoribonuclease Dicer homolog 1 (Gene Name=DCL1)