Structure of PDB 7elc Chain A

Receptor sequence

>7elcA (length=1409)

DEYVQELKGLIRKHRCEFGHQKSPLLTEGFKLLSSLVELESCEAHACQAN
TDQRFVDVILSDNGILCPTLPKVIPDGFKLTGKTLILLETFVRVNPDEFE
KKWKADMSKLLNLKHDLQKSGVTLVPIVDGRSNYNNRFVADWVIERIRWL
LIEILKASEDQEYQRLIHSLSNVKLENLEHLKRNSLDYDERLNESLFIGL
KGDIRESTVREELIKLKLWFKDEVFSKGLGKFKLTDRRELLESLSSLGAH
LDSDVSSCPFCNNKLMEIVYNVTFSCVERTDTHSNIEKHYLSVLSLCNKI
KGLKVFNTRRNTLLFLDLIMVNLMVDISDSCQDAIESLRKSGLIVGQMVM
LVNDRVLDILEAVKLIRKKIGTNPNWVKNCSKILERSHPEIWHHLSTLIK
QPDFNSLISIAQHLVSDRPIMRYSVKICRHKLFQEMSSFEQMRLFKTLSS
ISLSLINSMKTSFSSRLLVNEKYFGNVRLRECYAQRFYLAESLVGFLFYQ
KTGERSRCYSVYLSDNGVMSEQGSFYCDPKRFFLPVFSDEVLAGMCEEMT
SWLDFDTGLMNDTGPILRLLVLAILCSPSKRNQTFLQGLRYFLMAFANQI
HHIDLTSKLVVECKSSSEVVVQRLAVGLFIRLLSGESDASLFFSRRFKYL
LNVSYLCHLITKETPDRLTDQIKCFEKFIEPKVKFGCAVVNPSLNGKLTV
DQEDIMINGLKKFFSKSLRDTEDVQTPGVCKELLNYCVSLFNRGKLKVSG
ELKNNPFRSPTEFTSISSNSGNLKFGLSYKEQVGSNRELYVGDLNTKLMT
RLVEDFSEAVGNSMKYTCLNSEKEFERAICDMKMAVNNGDLSCSYDHSKW
GPTMSPALFLALLQMLELRTPVDRSKIDLDSVKSILKWHLHKVVEVPINV
AEAYCIGSTSLSEEFFHQTMQLNGQIPSHIMSVLDMGQGILHNTSDLYGL
ITEQFLCYALDLLYDVIPVSYTSSDDQITLIKTPSDAAEWLEMICFHEFL
SSKLNKFVSPKSVIGTFVAEFKSRFFVMGEETPLLTKFVAAALHNVKCKT
PTQLSETIDTICDQCIANGVSTKIVTRISKRVNQLIRYSGYGETPFGAIE
DQDVKDWVDGSRGYRLQRKIEAIFHDDKETSFIRNCARKVFNDIKRGRIF
EENLINLIGRGGDEALTGFLQYAGCSEQEVNRVLNYRWVNLSSFGDLRLV
LRVPTLIKTLQSKLSRQSSVASGFIGFCKSMGSKCVRDGKGGFLYIKEVY
SGVSACTCEICALKPKIIYCNNSLNKVSQFSKPILWDYFSLVLTNACELG
EWVFSTVKEPQNNQNFFWAVKPKVVRQIEDGMNHVLQSIRRNYPVLFDEH
LTPFMNDLQVSRLKFLDVCIALDMMNENLGIISHLLKTRDNSVYIVKQSD
CALAHIRQS

3D structure

• PDB: 7elc Cryo-EM structures of Lassa and Machupo virus polymerases complexed with cognate regulatory Z proteins identify targets for antivirals.
• Chain: A
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E324 S332 N335 K336 G339 K341 D391 L495 S496 K502 T503 S504 R532 F533 Y534 Y1449 K1623	E287 S295 N298 K299 G302 K304 D354 L453 S454 K460 T461 S462 R486 F487 Y488 Y1088 K1229
BS02	MN	A	D1188 D1330 E1381	D846 D976 E1020
BS03	ZN	A	C284 C287 C470 H472	C258 C261 C428 H430
BS04	ZN	A	C1650 C1655 C1664	C1256 C1261 C1270

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039689 negative stranded viral RNA replication
• GO:0039694 viral RNA genome replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044423 virion component

External links

• PDB: RCSB:7elc, PDBe:7elc, PDBj:7elc
• PDBsum: 7elc
• PubMed: 34127846
• UniProt: Q6IVU0