Structure of PDB 7el9 Chain A

Receptor sequence

>7el9A (length=1731)

DEYVQELKGLIRKHEFGHQKVTFLSQSPLLTEGFKLLSSLVELESCEAHA
CQADGFKLTGKTLILLETFVRVNPDEFEKKWKADMSKLLNLKHDLQKTLV
PIVDGRSNYNNRFVADWVIERIRWLLIEILKASEDQEYQRLIHSLSNVKN
QSLGLENLEHLKRNSLDYDERLNESLFIGLKGDIRESTVREELIKLKLWF
KDEVFSKGLGKFKLTDRRELLESLSSLGAHLDSDVSSCPFCNNKLMEIVY
NVTFSCVERTDTHSNIEKHYLSVLSLCNKIKGLKVFNTRRNTLLFLDLIM
VNLMVDISDSCQDAIESLRKSGLIVGQMVMLVNDRVLDILEAVKLIRKKI
GTNPNWVKNCSKILERSHPEIWHHLSTLIKQPDFNSLISIAQHLVSDRPI
MRYSVKICRHKLFQEMSSFEQMRLFKTLSSISLSLINSMKTSFSSRLLVN
EYFGNVRLRECYAQRFYLAESLVGFLFYQKTGERSRCYSVYLSDNGVMSE
QGSFYCDPKRFFLPVFSDEVLAGMCEEMTSWLDFDTGLMNDTGPILRLLV
LAILCSPSKRNQTFLQGLRYFLMAFANQIHHIDLTSKLVVECKSSSEVVV
QRLAVGLFIRLLSGESDASLFFSRRFKYLLNVSYLCHLITKETPDRLTDQ
IKCFEKFIEPKVKFGCAVVNPSLNGKLTVDQEDIMINGLKKFFSKSLRDT
EDVQTPGVCKELLNYCVSLFNRGKLKVSGELKNNPFRSPTEFTSISSNSG
NLKFGLSYKEQVGSNRELYVGDLNTKLMTRLVEDFSEAVGNSMKYTCLNS
EKEFERAICDMKMAVNNGDLSCSYDHSKWGPTMSPALFLALLQMLELRTP
VDRSKIDLDSVKSILKWHLHKVVEVPINVAEAYCIGTSLSEEFFHQTMQL
NGQIPSHIMSVLDMGQGILHNTSDLYGLITEQFLCYALDLLYDVIPVSYT
SSDDQITLIKTPSDAAEWLEMICFHEFLSSKLNKFVSPKSVIGTFVAEFK
SRFFVMGEETPLLTKFVAAALHNVKCKTPTQLSETIDTICDQCIANGVST
KIVTRISKRVNQLIRYSGYGETPFGAIEDQDVKDWVDGSRGYRLQRKIEA
IFHDDKETSFIRNCARKVFNDIKRGRIFEENLINLIGRGGDEALTGFLQY
AGCSEQEVNRVLNYRWVNLSSFGDLRLVEEVPTLIKTLQSKLSRQSSVAS
GFIGFCKSMGSKCVRDGKGGFLYIKEVYSGVSACTCEICALKPKIIYCNN
SLNKVSQFSKPILWDYFSLVLTNACELGEWVFSTVKEPQKPLNNQNFFWA
VKPKVVRQIEDQLGMNHVLQSIRRNYPVLFDEHLTPFMNDLQVSRTMDSG
RLKFLDVCIALDMMNENLGIISHLLKTRDNSVYIVKQSDCALAHIRQSSY
TDWELGLSPQQICTNFKTQLVLSSMVNPLVLSTSCLKSFFWFNEVLELED
DSQIELAELTDFALMVKNQNVSRAMFVEDIAMGYVVSNFEGVRISLSNVM
VDGVQLPPQDIGELFGLKAGLVVQIDHVRMSTKFKLKRKMVYSFSLECIM
DVGEIQNKEVILKVVAVDQSVSGSGGNHMLLDGVSVVASLPLFTGQASFD
LAAMLIESNLAGSNDNFLMRNVTLDLGGFSPELSDKYSYRLSGPENQEDP
LVLKDGAFYVGGERLSTYKVEFTGDLVVKALGALEDDESVVSMLHQLWPY
LKATSQVILFQQEDFTIVHDLYKKQLTKSIE

3D structure

• PDB: 7el9 Cryo-EM structures of Lassa and Machupo virus polymerases complexed with cognate regulatory Z proteins identify targets for antivirals.
• Chain: A
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E324 S332 N335 K336 N390 D391 S492 L495 S496 K502 R532 F533 Y534 Y1449 K1623 K1641	E267 S275 N278 K279 N333 D334 S430 L433 S434 K440 R465 F466 Y467 Y1066 K1207 K1225
BS02	MN	A	D1188 D1330 E1381	D825 D954 E998
BS03	ZN	A	C284 C287 C470 H472	C238 C241 C408 H410
BS04	ZN	A	C1650 C1655 C1664	C1234 C1239 C1248

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039689 negative stranded viral RNA replication
• GO:0039694 viral RNA genome replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044423 virion component

External links

• PDB: RCSB:7el9, PDBe:7el9, PDBj:7el9
• PDBsum: 7el9
• PubMed: 34127846
• UniProt: Q6IVU0