Structure of PDB 7e7q Chain A

Receptor sequence

>7e7qA (length=1958) Species: 9606 (Homo sapiens)

MGFVRQIQLLLWKNWTLRKRQKIRFVVELVWPLSLFLVLIWLRNANPLYS
HHECHFPNKAMPSAGMLPWLQGIFCNVNNPCFQSPTPGESPGIVSNYNNS
ILARVYRDFQELLMNAPESQHLGRIWTELHILSQFMDTLRTHPERIAGRG
IRIRDILKDEETLLLERFIIFSQSLSQGTLQWTLYANVDFFKLFRVLPTL
LDSRSQGINLRSWGGILSDMSPRIQEFIHRPSMQDLLWVTRPLMQNGGPE
TFTKLMGILSDLLCGYPEGGGSRVSYDRRTTSFCNALIQSLESNPLTKIA
WRAAKPLLMGKILYTPDSPAARRILKNANSTFEELEHVRKLVKAWEEVGP
QIWYFFDNSTQMNMIRDTLVKDFLNRQLGEEEAILNFLYKGPRESQAWRD
IFNITDRTLRLVNQYLECLVLDKFESYNDETQLTQRALSLLEENMFWAGV
VFPDMYPWTSSLPPHVKYKIRMDIDVVEKTNKIKDRYWDSGPRADPVEDF
RYIWGGFAYLQDMVEQGITRSQVQAEAPVGIYLQQMPYPCFVDDSFMIIL
NRCFPIFMVLAWIYSVSMTVKSIVLEKELRLKETLKNQGVSNAVIWCTWF
LDSFSIMSMSIFLLTIFIMHGRILHYSDPFILFLFLLAFSTATIMLCFLL
STFFSKASLAAACSGVIYFTLYLPHILCFAWQDRMTAELKKAVSLLSPVA
FGFGTEYLVRFEEQGLGLQWSNIGNSPTEGDEFSFLLSMQMMLLDAAVYG
LLAWYLDQVFPGDYGTPLPWYFLLQESDSFFEREHPGWVPGVCVKNLVKI
FEPCGRPAVDRLNITFYENQITAFLGHNGAGKTTTLSILTGLLPPTSGTV
LVGGRDIETSLDAVRQSLGMCPQHNILFHHLTVAEHMLFYAQLKGKSQEE
AQLEMEAMLEDTGLHHKRNEEAQDLSGGMQRKLSVAIAFVGDAKVVILDQ
PTSGVDPYSRRSIWDLLLKYRSGRTIIMSTHHMDEADLLGDRIAIIAQGR
LYCSGTPLFLKNCFGTGLYLTLVRKDVNELMDVVLHHVPEAKLVECIGQE
LIFLLPKHRAYASLFRELEETGLSSFGISDTPLEEIFLKVTEPECPGPQL
NTGTQLVLQHVQALLVKRFQHTIRSHKDFLAQIVLPATFVFLALMLSIVI
PPFGEYPALTLHPWIYGQQYTFFSMDEPGSEQFTVLADVLLNKPGFGNRC
LKEGWLPEYPCGNSTPWKTPSVSPNITQLFQKQKWTQVNPSPSCRCSTRE
KLTMLPECPEGAGGLPPPQRTQRSTEILQDLTDRNISDFLVKTYPALIRS
SLKSKFWVNEQRYGGISIGGKLPVVPITGEALVGFLSDLGRIMNVSGGPI
TREASKEIPDFLKHLETEDNIKVWFNNKGWHALVSFLNVAHNAILRASLP
KDRSPEEYGITVISQPLNLTKEQLSEITVLTTSVDAVVAICVIFSMSFVP
ASFVLYLIQERVNKSKHLQFISGVSPTTYWVTNFLWDIMNYSVSAGLVVG
IFIGFQKKAYTSPENLPALVALLLLYGWAVIPMMYPASFLFDVPSTAYVA
LSCANLFIGINSSAITFILELFENNRTLLRFNAVLRKLLIVFPHFCLGRG
LIDLALSQAVTDVYARFGEEHSANPFHWDLIGKNLFAMVVEGVVYFLLTL
LVQRHFFKTDILRLHELTKIYPGTSSPAVDRLCVGVRPGECFGLLGVNGA
GKTTTFKMLTGDTTVTSGDATVAGKSILTNISEVHQNMGYCPQFDAIDEL
LTGREHLYLYARLRGVPAEEIEKVANWSIKSLGLTVYADCLAGTYSGGNK
RKLSTAIALIGCPPLVLLDQPTTGMDPQARRMLWNVIVSIIREGRAVVLT
SHSMEECEALCTRLAIMVKGAFRCMGTIQHLKSKFGDGYIVTMKINPVEQ
FFQGNFPGSVQRERHYNMLQFQVSSSSLARIFQLLLSEEYSVTQTTLDQV
FVNFAKQQ

3D structure

• PDB: 7e7q Structural basis of substrate recognition and translocation by human ABCA4.
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 7.6.2.1: P-type phospholipid transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	F938 C941 A945 N965 G968 K969 T970 T971 Q1010 T2070 S2072	F801 C804 A808 N828 G831 K832 T833 T834 Q873 T1794 S1796
BS02	ATP	A	S1063 G1065 Y1947 N1974 G1977 K1978 T1979 T1980 Q2019	S926 G928 Y1671 N1698 G1701 K1702 T1703 T1704 Q1743
BS03	MG	A	T970 Q1010	T833 Q873
BS04	MG	A	T1979 Q2019	T1703 Q1743

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005501 retinoid binding
• GO:0005502 11-cis retinal binding
• GO:0005503 all-trans retinal binding
• GO:0005524 ATP binding
• GO:0005548 phospholipid transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0034632 retinol transmembrane transporter activity
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0090555 phosphatidylethanolamine flippase activity
• GO:0140326 ATPase-coupled intramembrane lipid transporter activity
• GO:0140327 flippase activity
• GO:0140347 N-retinylidene-phosphatidylethanolamine flippase activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0001523 retinoid metabolic process
• GO:0006649 phospholipid transfer to membrane
• GO:0006869 lipid transport
• GO:0007601 visual perception
• GO:0007603 phototransduction, visible light
• GO:0034633 retinol transport
• GO:0042574 retinal metabolic process
• GO:0045332 phospholipid translocation
• GO:0045494 photoreceptor cell maintenance
• GO:0055085 transmembrane transport

Cellular Component

• GO:0001750 photoreceptor outer segment
• GO:0005783 endoplasmic reticulum
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0031410 cytoplasmic vesicle
• GO:0042995 cell projection
• GO:0043231 intracellular membrane-bounded organelle
• GO:0097381 photoreceptor disc membrane
• GO:0120202 rod photoreceptor disc membrane

External links

• PDB: RCSB:7e7q, PDBe:7e7q, PDBj:7e7q
• PDBsum: 7e7q
• PubMed: 34158497
• UniProt: P78363|ABCA4_HUMAN Retinal-specific phospholipid-transporting ATPase ABCA4 (Gene Name=ABCA4)