Structure of PDB 7dn3 Chain A

Receptor sequence

>7dn3A (length=1293) Species: 9606 (Homo sapiens)

EQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLLY
GVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIG
ILQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKRGLKKKISDKCRKK
NICHHCGAFNGTVKKCGLLKIIHEAQENLNPLVVLNLFKRIPAEDVPLLL
MNPEAGKPSDLILTRLLVPPLCIRPDLTMKLTEIIFLNDVIKKHRISGAK
TQMIMEDWDFLQLQCALYINSELWTRGFVQRLKGKQGRFRGNLSGKRVDF
SGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVQNGPE
VHPGANFIQQRHTQMKRFLKYGNREKMAQELKYGDIVERHLIDGDVVLFN
RQPSLHKLSIMAHLARVKPHRTFRFNECVCTPYNADFDGDEMNLHLPQTE
EAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAK
ACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPV
RANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGSKNNI
FYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAK
YELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVIRDHA
GSACLRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGF
ENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLVDTAV
KTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAME
GKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFLCCQD
SFLQEIKKFIKGVSEKIKKTRDKYGINDNGTTEPRVLYQLDRITPTQVEK
FLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMNITLGVPRIKEIINA
SKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEVFLPDDC
FILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVT
PRENSKSSMYYVLQFLKEDLPKVVVQGIPEVSRAVIHIKYKLLVEGDNLR
AVMATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSID
RRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAY
FGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRP

3D structure

• PDB: 7dn3 Structure of human RNA polymerase III elongation complex.
• Chain: A
• Resolution: 3.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R353 R360 R366 Q465 P466 T865 G869 Y870 E1322 K1323	R290 R297 R303 Q402 P403 T802 G806 Y807 E1239 K1240
BS02	rna	A	R464 D503	R401 D440
BS03	MG	A	D499 D501 D503	D436 D438 D440
BS04	ZN	A	C72 H82	C69 H79

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0001056 RNA polymerase III activity
• GO:0003677 DNA binding
• GO:0003682 chromatin binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding
• GO:0071667 DNA/RNA hybrid binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0032728 positive regulation of interferon-beta production
• GO:0042797 tRNA transcription by RNA polymerase III
• GO:0045087 innate immune response
• GO:0051607 defense response to virus

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005666 RNA polymerase III complex
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane
• GO:0031981 nuclear lumen
• GO:0032991 protein-containing complex

External links

• PDB: RCSB:7dn3, PDBe:7dn3, PDBj:7dn3
• PDBsum: 7dn3
• PubMed: 33674783
• UniProt: O14802|RPC1_HUMAN DNA-directed RNA polymerase III subunit RPC1 (Gene Name=POLR3A)