Structure of PDB 7dfw Chain A

Receptor sequence

>7dfwA (length=957) Species: 9606 (Homo sapiens)

STHTLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVE
LWSAPNSQARSEKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKN
FSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTS
LYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDWKDHFLYCANAPLT
FKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNNYPA
GDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDEINRTTAE
DLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVM
AAMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEP
REVHIGRALGRVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAVI
LDFLLQMSAFVALLSLDSKRQEASRLDVCCCVKPQELPPPGQGEGLLLGF
FQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDS
YLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGMNAICSSAGCNNFSFT
QKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYISGPNKDKFCP
STVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAA
YSTSVNLTSDGQVLASRFMAYHKPLKNSQDYTEALRAARELAANITADLR
KVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLL
GLDLRSGLLNLLSIVMILVDTVGFMALWGISYNAVSLINLVSAVGMSVEF
VSHITRSFAISTKPTWLERAKEATISMGSAVFAGVAMTNLPGILVLGLAK
AQLIQIFFFRLNLLITLLGLLHGLVFLPVILSYVGPDVNPALALEQKRAE
EAVAAVM

3D structure

• PDB: 7dfw Structural insights into the mechanism of human NPC1L1-mediated cholesterol uptake.
• Chain: A
• Resolution: 2.69 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	P5S	A	Y646 A650 G652 Q722 G826 L827 L828 F832 F1213	Y315 A319 G321 Q391 G495 L496 L497 F501 F882
BS02	CLR	A	F1155 W1159 L1248	F824 W828 L917
BS03	CLR	A	E374 Y640 L677 F704	E43 Y309 L346 F373
BS04	CLR	A	W347 L358	W16 L27

Gene Ontology

Molecular Function

• GO:0005319 lipid transporter activity

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:7dfw, PDBe:7dfw, PDBj:7dfw
• PDBsum: 7dfw
• PubMed: 34272236
• UniProt: Q9UHC9|NPCL1_HUMAN NPC1-like intracellular cholesterol transporter 1 (Gene Name=NPC1L1)