Structure of PDB 7d3f Chain A

Receptor sequence

>7d3fA (length=1369) Species: 9606 (Homo sapiens)

QNPISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLGEPH
LPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAE
FLNIRIPPGDPMFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDPANQVTG
WLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQNPLLMWAAPDP
ATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWE
DEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSE
FVAASEQFLSTMVPPGVYMRNASCHFQGVINRNSSVSRALRVCNSYWSRE
HPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHLA
SCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAAL
YNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRN
GLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQL
STEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLME
ALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPVNFVLSRTLL
LKIPKEYDLVLLFNLEEERQALVENLRGALKESIQEWELREQELMRAAVT
REQRRHLLETFFRHLFSQVLSQKVREALTCELSRAEFAESLGLKPQDMFV
ESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGL
ISKDEFIRMLRSFIKAQLAEVVESELTWEDFHFMLLLFTEAHREKFQRSC
LHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGIT
DTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAA
VDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGS
EFPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHH
LYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEIS
VVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSA
PHEDTLSLHIRAAGPWTTRLREIYSAPTYPKLYLDGPFGEGHQEWHKFEV
SVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWL
ADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRS
LFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKA
CQLINRQDRTHFSHHYENF

3D structure

• PDB: 7d3f Structures of human dual oxidase 1 complex in low-calcium and high-calcium states.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 1.11.1.-
  1.6.3.1: NAD(P)H oxidase (H2O2-forming).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BMA	A	F1068 H1071	F892 H895
BS02	MAN	A	D61 V63 Y64	D40 V42 Y43
BS03	MAN	A	H1071 H1072	H895 H896
BS04	MAN	A	Y64 P119	Y43 P98
BS05	MAN	A	Y64 E121	Y43 E100
BS06	HEM	A	F1097 M1104 R1106 H1130 R1131 A1134 M1205 Y1206 A1209 F1221 W1222 H1225 Y1228 Y1260 E1317	F921 M928 R930 H954 R955 A958 M1029 Y1030 A1033 F1045 W1046 H1049 Y1052 Y1084 E1141
BS07	HEM	A	A1090 I1093 F1097 H1144 H1148 P1191 G1195 L1199 L1202 L1235 H1238 G1239 A1242 L1243 I1244 P1247 R1248 F1249	A914 I917 F921 H968 H972 P1015 G1019 L1023 L1026 L1059 H1062 G1063 A1066 L1067 I1068 P1071 R1072 F1073
BS08	NDP	A	R1036 E1039 N1040 Y1041 G1389 I1390 G1391 T1423 R1424 T1455 R1495 G1521 P1522 G1524	R860 E863 N864 Y865 G1207 I1208 G1209 T1241 R1242 T1273 R1313 G1339 P1340 G1342
BS09	FAD	A	D1128 R1214 W1305 H1319 P1320 F1321 T1322 H1335 I1336 R1337 G1340 P1341 W1342 T1343	D952 R1038 W1129 H1143 P1144 F1145 T1146 H1159 I1160 R1161 G1164 P1165 W1166 T1167
BS10	CA	A	D830 N832 Y834 E839	D760 N762 Y764 E769
BS11	CA	A	D864 D866 G867 N868 L870	D794 D796 G797 N798 L800

Gene Ontology

Molecular Function

• GO:0004601 peroxidase activity
• GO:0005509 calcium ion binding
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding

Biological Process

• GO:0006979 response to oxidative stress

External links

• PDB: RCSB:7d3f, PDBe:7d3f, PDBj:7d3f
• PDBsum: 7d3f
• PubMed: 33420071
• UniProt: Q9NRD9|DUOX1_HUMAN Dual oxidase 1 (Gene Name=DUOX1)