Structure of PDB 7cug Chain A

Receptor sequence
>7cugA (length=303) Species: 36824 (Bacillus sp. TB-90) [Search protein sequence]
VMYYGKGDVFAYRTYLKPLTGVRTIPESPFSGRDHILFGVNVKISVGGTK
LLTSFTKGDNSLVVATDSMKNFIQKHLASYTGTTIEGFLEYVATSFLKKY
SHIEKISLIGEEIPFETTFAVKNGNRAASELVFKKSRNEYATAYLNMVRN
EDNTLNITEQQSGLAGLQLIKVSGNSFVGFIRDEYTTLPEDSNRPLFVYL
NIKWKYKNTEDSFGTNPENYVAAEQIRDIATSVFHETETLSIQHLIYLIG
RRILERFPQLQEVYFESQNHTWDKIVEEIPESEGKVYTEPRPPYGFQCFT
VTQ
3D structure
PDB7cug Identification of quasi-stable water molecules near the Thr73-Lys13 catalytic diad of Bacillus sp. TB-90 urate oxidase by X-ray crystallography with controlled humidity.
ChainA
Resolution1.62 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K13 T73 R201 Q250 T278
Catalytic site (residue number reindexed from 1) K6 T66 R194 Q243 T271
Enzyme Commision number 1.7.3.3: factor independent urate hydroxylase.
4.1.1.97: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 AZA A F184 R201 S248 I249 Q250 F177 R194 S241 I242 Q243
BS02 MXE A E119 E146 E112 E139
BS03 MXE A Y10 S52 V53 K112 Y3 S45 V46 K105
BS04 MXE A I43 L44 F140 I36 L37 F133
BS05 AZA A A72 T73 A65 T66
Gene Ontology
Molecular Function
GO:0004846 urate oxidase activity
Biological Process
GO:0006144 purine nucleobase metabolic process

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:7cug, PDBe:7cug, PDBj:7cug
PDBsum7cug
PubMed33002140
UniProtQ45697|PUCL_BACSB Uric acid degradation bifunctional protein (Gene Name=uao)

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