Structure of PDB 7cu3 Chain A

Receptor sequence

>7cu3A (length=1277) Species: 10116 (Rattus norvegicus)

LWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMF
LYTAEMIAKMHIRGIDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPW
GMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFL
LLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQC
PPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRA
IDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWPAC
LQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFT
VLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTY
FQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQ
MFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVA
IYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQRSILSVQHHI
RQERREHRFRNFCRVVVRARFTKYHQLYDLLGLVTYLDWVMITVTICSCI
SMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIR
DFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVP
QMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNI
IRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNV
GNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLF
VGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRA
KMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFI
FVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYM
MGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAF
AGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQ
PPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFS
LFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRL
EVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLE
ELLAREQLEYTIEEEVAKQTIRMWLKK

3D structure

• PDB: 7cu3 Structure of voltage-modulated sodium-selective NALCN-FAM155A channel complex.
• Chain: A
• Resolution: 2.65 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	6OU	A	F953 G954 F997 V1007 R1008 F1011 L1345 I1433	F652 G653 F696 V706 R707 F710 L1044 I1132
BS02	6OU	A	L1025 G1102 N1103 F1109 Y1420 M1425 V1432	L724 G801 N802 F808 Y1119 M1124 V1131
BS03	6OU	A	F307 D880 L884 Q1002 M1332 F1336 F1337	F268 D579 L583 Q701 M1031 F1035 F1036
BS04	6OU	A	Y157 G507 S508 V511	Y118 G431 S432 V435
BS05	6OU	A	I303 K1377 T1380	I264 K1076 T1079
BS06	6OU	A	D105 R106 W107 F110 F158 F585	D66 R67 W68 F71 F119 F509
BS07	6OU	A	Y64 I518 F540	Y34 I442 F464
BS08	6OU	A	P541 F544 M545 P1130 G1133 F1141	P465 F468 M469 P829 G832 F840
BS09	6OU	A	S403 Y405 N1100 V1101	S327 Y329 N799 V800
BS10	6OU	A	L485 I486 I488 S489 G501 I1022 L1024 T1026	L409 I410 I412 S413 G425 I721 L723 T725
BS11	6OU	A	S1271 R1272 R1273 Y1276 K1322	S970 R971 R972 Y975 K1021
BS12	CLR	A	Y415 P467	Y339 P391

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005248 voltage-gated sodium channel activity
• GO:0005261 monoatomic cation channel activity
• GO:0005272 sodium channel activity
• GO:0022840 leak channel activity

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0006814 sodium ion transport
• GO:0008150 biological_process
• GO:0030001 metal ion transport
• GO:0032224 positive regulation of synaptic transmission, cholinergic
• GO:0032230 positive regulation of synaptic transmission, GABAergic
• GO:0034220 monoatomic ion transmembrane transport
• GO:0035725 sodium ion transmembrane transport
• GO:0055085 transmembrane transport
• GO:0060075 regulation of resting membrane potential
• GO:0070588 calcium ion transmembrane transport
• GO:0071805 potassium ion transmembrane transport
• GO:0098655 monoatomic cation transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0034702 monoatomic ion channel complex

External links

• PDB: RCSB:7cu3, PDBe:7cu3, PDBj:7cu3
• PDBsum: 7cu3
• PubMed: 33273469
• UniProt: Q6Q760|NALCN_RAT Sodium leak channel NALCN (Gene Name=Nalcn)