Structure of PDB 7ctt Chain A

Receptor sequence

>7cttA (length=826) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

VYRAFDIYNDKVAGFAKFLKETIYNLLKDCPAVAKHDRLTKYTMADLVYA
LRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGE
RVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSG
VPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERL
KLFDRYFKYWDQTYHPNCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLV
RKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAM
HAASGNLLLDKRTTCFSVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFK
EGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYF
DCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAY
TKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIA
ATRGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNML
RIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGT
SSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHRLYEC
LYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIK
NFKSVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLP
YPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADV
FHLYLQYIRKLHYWEPEFYEAMYTPH

3D structure

• PDB: 7ctt Structural Basis of SARS-CoV-2 Polymerase Inhibition by Favipiravir.
• Chain: A
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R513 S759 C813 S814 Q815 R836 I847 R858 S861 L862 D865	R433 S679 C733 S734 Q735 R756 I767 R778 S781 L782 D785
BS02	rna	A	K500 S501 N507 V557 G559 R569 K577 G590 S592 F594 Y595 G683 D684 A685 Y689 Y915 F920 M924	K420 S421 N427 V477 G479 R489 K497 G510 S512 F514 Y515 G603 D604 A605 Y609 Y813 F818 M822
BS03	ZN	A	H295 C301 C306	H215 C221 C226
BS04	ZN	A	C487 H642 C646	C407 H562 C566
BS05	GE6	A	R555 D618 D623 S682 D760 K798	R475 D538 D543 S602 D680 K718

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:7ctt, PDBe:7ctt, PDBj:7ctt
• PDBsum: 7ctt
• PubMed: 33521757
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)