Structure of PDB 7c7l Chain A

Receptor sequence

>7c7lA (length=503) Species: 115547 (uncultured archaeon) [Search protein sequence]

KNTITKTLKLRIVRPYNSAEVEKIVADEKNNREKIALKEACSKHLKVTTQ
VERNACLFCKARKLDDKFYQKLRGQFPDAVFWQEISEIFRQLQKQAAEIY
NQSLIELYYEIFIKGKGIANASSVEHYLSDVCYTRAAELFKNAAIASGLR
SKIKSNFRLKELKNMKSGLPTTKSDNFPIPLVKQKGGQYTGFEISNHNSD
FIIKIPFGRWQVKKEIDKYRPWEKFDFEQVQKSPKPISLLLSTQRRKRNK
GWSKDEGTEAEIKKVMNGDYQTSYIEVKRGSKIGEKSAWMLNLSIDVPKI
DKGVDPSIIGGIAVGVKSPLVCAINNAFSRYSISDNDLFHFNKKMFARRR
ILLKKNRHKRAGHGAKNKLKPITILTEKSERFRKKLIERWACEIADFFIK
NKVGTVQMENLESMKRKEDSYFNIRLWPYAEMQNKIEFKLKQYGIEIRKV
APNNTSKTCSKCGHLNNYFNFEYRKKNKFPHFKCEKCNADYNAALNISNP
KLK

3D structure

PDB

7c7l Structure of the miniature type V-F CRISPR-Cas effector enzyme.

Chain

Resolution

3.3 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	A	T7 K8 T9 K11 R13 R16 K31 R66 N67 C72 K76 Y82 W95 S99 Y113 K165 N169 S180 P183 T185 P191 S255 T256 Q257 R258 R259 T271 E272 E289 K291 K299 N305 R363 L366 K367 K368 K372 R373 A374 G375 H376 G377 A378 K379 K381 L382 K391 R394 K397 K398 E401 C405 F454 K455	T5 K6 T7 K9 R11 R14 K29 R53 N54 C59 K63 Y69 W82 S86 Y100 K152 N156 S167 P170 T172 P178 S242 T243 Q244 R245 R246 T258 E259 E276 K278 K286 N292 R350 L353 K354 K355 K359 R360 A361 G362 H363 G364 A365 K366 K368 L369 K378 R381 K384 K385 E388 C392 F438 K439
BS02	dna	A	T7 K167 S168 R171 Q197 K198 Y202 S286 L382 L424 K428 W443 P444 Y445 A446	T5 K154 S155 R158 Q184 K185 Y189 S273 L369 L411 K415 W427 P428 Y429 A430
BS03	dna	A	I131 N133 S135 S136 H139 Y140 S142 Y146 A156 R163 K173 K176 K179 Q242 V243 Q244	I118 N120 S122 S123 H126 Y127 S129 Y133 A143 R150 K160 K163 K166 Q229 V230 Q231
BS04	ZN	A	C50 H53 C69 C72	C41 H44 C56 C59
BS05	ZN	A	C475 C478 C500 C503	C459 C462 C484 C487

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0051607	defense response to virus

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:7c7l, PDBe:7c7l, PDBj:7c7l
PDBsum	7c7l
PubMed	33333018
UniProt	A0A482D308\|CS12F_UNCAX CRISPR-associated endodeoxyribonuclease Cas12f1 (Gene Name=cas12f)

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