Structure of PDB 7c49 Chain A

Receptor sequence

>7c49A (length=434) Species: 1042876 (Pseudomonas putida S16)

KGGFDYDVVVVGGGFAGATAARECGLQGYRTLLLEARSRLGGRTFTSRFA
GQEIEFGGAWVHWLQPHVWAEMQRYGLGVVEDPLTNLDKTLIMYNDGSVE
SISPDEFGKNIRIAFEKLCHDAWEVFPRPHEPMFTERARELDKSSVLDRI
KTLGLSRLQQAQINSYMALYAGETTDKFGLPGVLKLFACGGWNYDAFMDT
ETHYRIQGGTIGLINAMLTDSGAEVRMSVPVTAVEQVNGGVKIKTDDDEI
ITAGVVVMTVPLNTYKHIGFTPALSKGKQRFIKEGQLSKGAKLYVHVKQN
LGRVFAFADEQQPLNWVQTHDYSDELGTILSITIARKETIDVNDRDAVTR
EVQKMFPGVEVLGTAAYDWTADPFSLGAWAAYGVGQLSRLKDLQAAEGRI
LFAGAETSNGWHANIDGAVESGLRAGREVKQLLS

3D structure

• PDB: 7c49 Molecular Deceleration Regulates Toxicant Release to Prevent Cell Damage in Pseudomonas putida S16 (DSM 28022).
• Chain: A
• Resolution: 2.25 Å

Enzymatic activity

• Enzyme Commision number: 1.4.2.2: nicotine dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	V59 G60 G62 A64 E83 A84 R85 G90 R91 T92 G105 G106 A107 W108 P278 V279 V308 P309 W417 F422 A426 W427 G452 A453 A461 N462 I463 A466	V11 G12 G14 A16 E35 A36 R37 G42 R43 T44 G57 G58 A59 W60 P230 V231 V260 P261 W369 F374 A378 W379 G404 A405 A413 N414 I415 A418
BS02	HNL	A	L217 Y218 W427 A461	L169 Y170 W379 A413

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity

External links

• PDB: RCSB:7c49, PDBe:7c49, PDBj:7c49
• PDBsum: 7c49
• PubMed: 32873764
• UniProt: F8G0P2|NICA2_PSEP6 Nicotine dehydrogenase (Gene Name=nicA2)