Structure of PDB 7bkq Chain A

Receptor sequence

>7bkqA (length=640) Species: 10090 (Mus musculus)

LQLRPYQMEVAQPALDGKNIIICLPTGSGKTRVAVYITKDHLDKKKQASE
SGKVIVLVNKVMLAEQLFRKEFNPYLKKWYRIIGLSGDTQLKISFPEVVK
SYDVIISTAQILENSLLNLESGDDDGVQLSDFSLIIIDECHHTNKEAVYN
NIMRRYLKQKLRNNDLKKQNKPAIPLPQILGLTASPGVGAAKKQSEAEKH
ILNICANLDAFTIKTVKENLGQLKHQIKKFVIANPFKEKLLEIMASIQTY
CQKSPMSDFGTQHYEQWAIQMEKKAAKDGNRKDRVCAEHLRKYNEALQIN
DTIRMIDAYSHLETFYTDEKEKKFAVLNDKLDETDEFLMNLFFDNKKMLK
KLAENPEKLIKLRNTILEQFTRSSSRGIIFTKTRQSTYALSQWIMENAKF
AEVGVKAHHLIGAGHSSEVKPMTQTEQKEVISKFRTGEINLLIATTVAEE
GLDIKECNIVIRYGLSTYVLVTEREIVNDFREKMMYKAINRVQNMKPEEY
AHKILELQVQSILEKKMKVKRSIAKQYNDNPSLITLLCKNCSMLVCSGEN
IHVIEKMHHVNMTPEFKGLYIVRENKNGEIICKCGQAWGTMMVHKGLDLP
CLKIRNFVVNFKNNSPKKQYKKWVELPIRFPDLDYSEYCL

3D structure

• PDB: 7bkq MDA5 disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA.
• Chain: A
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K451 E452 Q581 T767 T769 H927 K1002	K145 E146 Q266 T423 T425 H558 K622
BS02	rna	A	N365 V367 G393 I417 E580 Q581 K726 R728 I755 G756 S761 T789 T790 V791 H974 K1001	N59 V61 G87 I111 E265 Q266 K382 R384 I411 G412 S417 T445 T446 V447 H594 K621
BS03	ZN	A	C907 C962 C964	C538 C582 C584
BS04	ADP	A	R310 G335 K336 T337 R338	R4 G29 K30 T31 R32

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity

External links

• PDB: RCSB:7bkq, PDBe:7bkq, PDBj:7bkq
• PDBsum: 7bkq
• PubMed: 34795277
• UniProt: Q8R5F7|IFIH1_MOUSE Interferon-induced helicase C domain-containing protein 1 (Gene Name=Ifih1)