Structure of PDB 7aml Chain A

Receptor sequence

>7amlA (length=596) Species: 7955 (Danio rerio)

GLYFPQRLYTENIYVGQQQGSPLLQVISMREFPTERPYFFLCSHRDAFTS
WFHIDEASGVLYLNKTLEWSDFSSLRSGSVRSPKDLTLKVGVSSTPPMKV
MCTILPTVEVKLSFINDTAPSCGQVELSTLCFPEKISNPHITENREPGAL
RQLRRFTHMSICPNYTISYGVVAGSSVPFAVDDSTSELVVTAQVDREEKE
VYHLDIVCMVRTERNLEEVFRSLHVNIYDEDDNSPYVNGTDTEDVLVEFD
RSEGTVFGTLFVYDRDTTPVYPTNQVQNKLVGTLMTNDSWIKNNFAIEHK
FREEKAIFGNVRGTVHEYKLKLSQNLSVTEQRSFLLGYLVNDTTFPGPEG
TVLLHFNVTVLPVPIRFSNVTYSFTVSQKATTYSQIGKVCVENCQKFKGI
DVTYQLEIVDRNITAEAQSCYWAVSLAQNPNDNTGVLYVNDTKVLRRPEC
QELEYVVIAQEQQNKLQAKTQLTVSFQGEADSLRTDEPRFPACAEKRQRG
DCEATRGLGAPTGRCQWRQGRDKGISKRYSTCSPNLGTCPDGYCDAIESK
NISICPQDCSSEAIIGGYERDLYGIKAGHGTCYCFEGKCFCERDEP

3D structure

• PDB: 7aml A two-site flexible clamp mechanism for RET-GDNF-GFR alpha 1 assembly reveals both conformational adaptation and strict geometric spacing.
• Chain: A
• Resolution: 3.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.10.1: receptor protein-tyrosine kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	E164 N165 D216 E218 D253	E143 N144 D195 E197 D232
BS02	CA	A	E164 E218 D250 E251 D253 D287	E143 E197 D229 E230 D232 D266
BS03	CA	A	D252 N254 D285 D287 N299 D363	D231 N233 D264 D266 N278 D342
BS04	CA	A	T559 Y564 C565 D566 E569 D579	T538 Y543 C544 D545 E548 D558

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding

Biological Process

• GO:0007156 homophilic cell adhesion via plasma membrane adhesion molecules

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:7aml, PDBe:7aml, PDBj:7aml
• PDBsum: 7aml
• PubMed: 33484636
• UniProt: A8E7C6