Structure of PDB 7aea Chain A

Receptor sequence

>7aeaA (length=1381) Species: 9606 (Homo sapiens)

KEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLL
YGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVI
GILQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKRGLKKKISDKCRK
KNICHHCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIE
HNKEVEPLLGRAQENLNPLVVLNLFKRIPAEDVPLLLMNPEAGKPSDLIL
TRLLVPPLCIRPSVVNEDDLTMKLTEIIFLNDVIKKHRISGAKTQMIMED
WDFLQLQCALYINSELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGK
RVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVQ
NGPEVHPGANFIQQRHTQMKRFLKYGNREKMAQELKYGDIVERHLIDGDV
VLFNRQPSLHKLSIMAHLARVKPHRTFRFNECVCTPYNADFDGDEMNLHL
PQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFF
DRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSD
DNPVRANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGS
KNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGL
LKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVI
RDHAGSACLRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRV
PDGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLV
DTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDP
AAMEGKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFL
CCQDSFLQEIKKFIKGVSEKIKKTRDKYGINDNGTTEPRVLYQLDRITPT
QVEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVA
SMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTL
LGEISEYIEEVFLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLR
VKPGDVAVHGEAVVCVTPRENSKSSMYYVLQFLKEDLPKVVVQGIPEVSR
AVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLG
IEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLA
KMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGL
FKLLHKADRDPNPPKRPLIFDTNEFHIPLVT

3D structure

• PDB: 7aea Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states.
• Chain: A
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R464 G502 D503	R455 G493 D494
BS02	dna	A	K167 R1159 F1306	K165 R1150 F1297
BS03	dna	A	S326 R353 R360 R366 Q465 E504 T865 Y870 E1322 K1323	S317 R344 R351 R357 Q456 E495 T856 Y861 E1313 K1314
BS04	ZN	A	C69 C72	C67 C70
BS05	ZN	A	C109 C156	C107 C154
BS06	MG	A	D499 D501 D503	D490 D492 D494

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0001056 RNA polymerase III activity
• GO:0003677 DNA binding
• GO:0003682 chromatin binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding
• GO:0071667 DNA/RNA hybrid binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0032728 positive regulation of interferon-beta production
• GO:0042797 tRNA transcription by RNA polymerase III
• GO:0045087 innate immune response
• GO:0051607 defense response to virus

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005666 RNA polymerase III complex
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane
• GO:0031981 nuclear lumen
• GO:0032991 protein-containing complex

External links

• PDB: RCSB:7aea, PDBe:7aea, PDBj:7aea
• PDBsum: 7aea
• PubMed: 33558764
• UniProt: O14802|RPC1_HUMAN DNA-directed RNA polymerase III subunit RPC1 (Gene Name=POLR3A)