Structure of PDB 7ae1 Chain A

Receptor sequence
>7ae1A (length=1381) Species: 9606 (Homo sapiens) [Search protein sequence]
KEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLL
YGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVI
GILQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKRGLKKKISDKCRK
KNICHHCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIE
HNKEVEPLLGRAQENLNPLVVLNLFKRIPAEDVPLLLMNPEAGKPSDLIL
TRLLVPPLCIRPSVVNEDDLTMKLTEIIFLNDVIKKHRISGAKTQMIMED
WDFLQLQCALYINSELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGK
RVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVQ
NGPEVHPGANFIQQRHTQMKRFLKYGNREKMAQELKYGDIVERHLIDGDV
VLFNRQPSLHKLSIMAHLARVKPHRTFRFNECVCTPYNADFDGDEMNLHL
PQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFF
DRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSD
DNPVRANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGS
KNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGL
LKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVI
RDHAGSACLRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRV
PDGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLV
DTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDP
AAMEGKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFL
CCQDSFLQEIKKFIKGVSEKIKKTRDKYGINDNGTTEPRVLYQLDRITPT
QVEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVA
SMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTL
LGEISEYIEEVFLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLR
VKPGDVAVHGEAVVCVTPRENSKSSMYYVLQFLKEDLPKVVVQGIPEVSR
AVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLG
IEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLA
KMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGL
FKLLHKADRDPNPPKRPLIFDTNEFHIPLVT
3D structure
PDB7ae1 Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states.
ChainA
Resolution2.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna A R464 D503 R455 D494
BS02 dna A K167 R1159 K165 R1150
BS03 dna A S326 K348 R353 R360 R366 T865 Y870 E1322 S317 K339 R344 R351 R357 T856 Y861 E1313
BS04 ZN A C69 H82 C67 H80
BS05 ZN A C109 C156 C159 C107 C154 C157
BS06 MG A D499 D501 D503 D490 D492 D494
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0001056 RNA polymerase III activity
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
GO:0071667 DNA/RNA hybrid binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0032728 positive regulation of interferon-beta production
GO:0042797 tRNA transcription by RNA polymerase III
GO:0045087 innate immune response
GO:0051607 defense response to virus
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005666 RNA polymerase III complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane
GO:0031981 nuclear lumen
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7ae1, PDBe:7ae1, PDBj:7ae1
PDBsum7ae1
PubMed33558764
UniProtO14802|RPC1_HUMAN DNA-directed RNA polymerase III subunit RPC1 (Gene Name=POLR3A)

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