Structure of PDB 6z8k Chain A

Receptor sequence

>6z8kA (length=2158)

MDYQEYQQFLARINTARDACVAKDIDVDLLMARHDYFGRELCKSLNIEYR
NDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSNE
SSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELYP
TIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCPE
FWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKKD
YSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLHD
QKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGKM
MDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQFM
INNDLIDKSEKLKLFKNFCGIGKSKPKILDFDDANMYLASLTMMEQSKKI
LSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDFSTL
MKNILSVSQYNRHNTFRIAMCANNNVFAIVFPATVVYSIIVLHKEEENIF
NPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLFLTTCLLFKHDN
PTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLAISSNVKDYIAE
KFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYLSDYDITQKGIK
DNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHHVMVDLAKTILE
IECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADTSRHNHLRNRIE
NRNNFRRSITTISTFTSSKSCLKIGDFRKEKELQSVKQKKILEVQSRKNY
EMLRNAMPNYTDYISTKVFDRLYELLDKKVLTDKPVIEQIMDMMIDHKKF
YFTFFNKGQKTSKDREIFVGEYEAKMCMYAVERIAKERCKLNPDEMISEP
GDGKLKVLEQKSEQEIRFLVETTRQKNREIDEAIELGKIEKLSLGKAKGL
KMEINADMSKWSAQDVFYKYFWLIALDPILYPQEKERILYFMCNYMDKEL
ILPDELLFNLLDQKVAYQNDIIATMTNQLNSNTVLIKRNWLQGNFNYTSS
YVHSCAMSVYKEILKEAITLLDGSILVNSLVHSDDNQTSITIVQDKMEND
KIIDFAMKEFERACLTFGCQANMKKTYVTNCIKEFVSLFNLYGEPFSIYG
RFLLTSVGDCAYIGPYEDLASRISSAQTAIKHGCPPSLAWVSIAISHWMT
SLTYNMLPGQSNDPIDYFPAENRKDIPIELNGVLDAPLSMISTVGLESGN
LYFLIKLLSKYTPVMQKRESVVNQIAEVKNWKVEDLTDNEIFRLKILRYL
VLDAEMDPSDIMGETSDMRGRSILTPRKFTTAGSLRKLYSFSKYQDRLSS
PGGMVELFTYLLEKPELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQNP
AQLFIEQILFSHKPVIDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLELT
NDDIQVIYSYIILNDPMMITIANTHILSIYGSPQRRMGMSCSTMPEFRNL
KLIHHSPALVLRAYSKNNPDPTEMARDLVHLKEFVENTNLEEKMKVRIAM
NEAEKGQRDIVFELKEMTRFYQVCYEYVKSHKIKVFILPAKSYTTTDFCS
LMQGNLIKDKEWYTVHYLKQILSGGHKAIMQHNATSEQNIAFECFKLITH
FADSFIDSLSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLF
RTGDLRQADLDKYDAMKSHERVTWNDWINLTITGYNRSITIIGEDNKLTY
AELCLTRKTPENITISGRKLLGSRHGLKFENMSKIQNYYITYRKKDRHQF
VYQIHSHESITRRNEEHMAIRTRIYNEITPVCVVNVAEVDGDQRILIRSL
DYLNNDIFSLSRIKVGLDEFATIKKAHFSKMVSFEGPPIKTGLLDLTELM
KSQDLLNLNYDNIRNSNLISFSKLICCEGSDNINDGLEFLSDDPMNFTEG
EAIHSTPIFNIYYSKRGERHMTYRNAIKLLIERETKIFEEAFTFSENGFI
SPENLGCLEAVVSLIKLLKTNEWSTVIDKCIHICLIKNGMDHMYHSFDVP
KCFMGNPITRDINWVMFREFINSLPGTDIPPWNVMTENFKKKCIALINSK
FSEFTKLM

3D structure

• PDB: 6z8k Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
• Chain: A
• Resolution: 3.02 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Q291 R292 K302 C419 G420 K439 P440 R592 A593 R595 R600 T642 K643 Y677 E758 H760 H761 K768 L1113 Q1116 V1118 Y1120 D1123	Q291 R292 K302 C419 G420 K425 P426 R570 A571 R573 R578 T620 K621 Y655 E736 H738 H739 K746 L1060 Q1063 V1065 Y1067 D1070
BS02	rna	A	H312 N313 N318 K368 R372 I378 K381 K382 L383 W395 E396 L471 K472 N517 S520 V521 Y524 R526 R531 C535 A536 N537 N538 L1512 H1515 K1516	H312 N313 N318 K368 R372 I378 K381 K382 L383 W395 E396 L457 K458 N503 S506 V507 Y510 R512 R517 C521 A522 N523 N524 L1459 H1462 K1463
BS03	rna	A	K841 I960 F961 V962 R976 P993 G994 G1146 E1270 A1273 S1425 K1705 I1706 K1707	K819 I917 F918 V919 R933 P950 G951 G1093 E1217 A1220 S1372 K1632 I1633 K1634
BS04	rna	A	Y750 S840 R958 Q1145 G1146 H1185 S1186 D1187 V1239 S1240 R1254 D1262 A1264 V1472 K1474 S1497	Y728 S818 R915 Q1092 G1093 H1132 S1133 D1134 V1186 S1187 R1201 D1209 A1211 V1419 K1421 S1444
BS05	ZN	A	C2064 H2169 D2178 H2182	C1977 H2082 D2091 H2095
BS06	MG	A	D1188 E1237	D1135 E1184

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:6z8k, PDBe:6z8k, PDBj:6z8k
• PDBsum: 6z8k
• PubMed: 32681014
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)