Structure of PDB 6ycn Chain A

Receptor sequence

>6ycnA (length=403) Species: 385957 (Amycolatopsis sp. ATCC 39116)

ERPDLAWLDEVTMTQLERNPYEVYERLRAEAPLAFVPVLGSYVASTAEVC
REVATSPDFEAVITPAGGRTFGHPAIIGVNGDIHADLRSMVEPALQPAEV
DRWIDDLVRPIARRYLERFENDGHAELVAQYCEPVSVRSLGDLLGLQEVD
SDKLREWFAKLNRSATNAAVDENGEFANPEGFAEGDQAKAEIRAVVDPLI
DKWIEHPDDSAISHWLHDGMPPGQTRDREYIYPTIYVYLLGAMQEPGHGM
ASTLVGLFSRPEQLEEVVDDPTLIPRAIAEGLRWTSPIWSATARISTKPV
TIAGVDLPAGTPVMLSYGSANHDTGKYEAPSQYDLHRPPLPHLAFGAGNH
ACAGIYFANHVMRIALEELFEAIPNLERDTREGVEFWGWGFRGPTSLHVT
WEV

3D structure

• PDB: 6ycn Engineering a Cytochrome P450 for Demethylation of Lignin-Derived Aromatic Aldehydes.
• Chain: A
• Resolution: 1.83 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R167 Q248 E249 C356 A357 G358
• Catalytic site (residue number reindexed from 1): R163 Q244 E245 C352 A353 G354
• Enzyme Commision number: 1.14.14.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	I81 H88 R92 Y242 A246 E249 I292 T296 R298 Y321 A348 F349 G350 A351 H354 C356 F361 A362 M366	I77 H84 R88 Y238 A242 E245 I288 T292 R294 Y317 A344 F345 G346 A347 H350 C352 F357 A358 M362
BS02	OKE	A	F75 I81 V241 G245 A246 I292 T296 F395	F71 I77 V237 G241 A242 I288 T292 F391

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding
• GO:0046872 metal ion binding

Biological Process

• GO:0009056 catabolic process

External links

• PDB: RCSB:6ycn, PDBe:6ycn, PDBj:6ycn
• PDBsum: 6ycn
• PubMed: 34467290
• UniProt: P0DPQ7|GCOA_AMYS7 Aromatic O-demethylase, cytochrome P450 subunit (Gene Name=gcoA)