Structure of PDB 6x74 Chain A

Receptor sequence

>6x74A (length=439) Species: 4932 (Saccharomyces cerevisiae)

IVACDDPDFLTSYFAHSRLHHLSAWKANLKDKFLNENIHKYTKITDKDTY
IIFHIDFDCFFATVAYLCRSSSFSACDFKRDPIVVCHGTKNSDIASCNYV
ARSYGIKNGMWVSQAEKMLPNGIKLISLPYTFEQFQLKSEAFYSTLKRLN
IFNLILPISIDEAVCVRIIPDNIHNTNTLNARLCEEIRQEIFQGTNGCTV
SIGCSDSLVLARLALKMAKPNGYNITFKSNLSEEFWSSFKLDDLPGVGHS
TLSRLESTFDSPHSLNDLRKRYTLDALKASVGSKLGMKIHLALQGQDDEE
SLKILYDPKEVLQRKSLSIDINWGIRFKNITQVDLFIERGCQYLLEKLNE
INKTTSQITLKLMRRCKDAPIEPPKYMGMGRCDSFSRSSRLGIPTNEFGI
IATEMKSLYRTLGCPPMELRGLALQFNKLVDVGPDNNQL

3D structure

• PDB: 6x74 Visualizing Rev1 catalyze protein-template DNA synthesis.
• Chain: A
• Resolution: 1.69 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R324 D362 F366 F367 A401 S402 D467 E468 R518 P551 G552 G554 H555 S556 T557 F691 S692 R693 S694 R696	R18 D56 F60 F61 A95 S96 D161 E162 R212 P245 G246 G248 H249 S250 T251 F385 S386 R387 S388 R390
BS02	dna	A	Y319 F320 H322 S323 R324 L325 H326 S329 H393 G394 T395 K396 N397 S398 D399 S589 K590 R620 K621 S622 S624 I625 D626 N628 W629 K681 Y682 G684 M685 G686	Y13 F14 H16 S17 R18 L19 H20 S23 H87 G88 T89 K90 N91 S92 D93 S283 K284 R314 K315 S316 S318 I319 D320 N322 W323 K375 Y376 G378 M379 G380
BS03	MG	A	D548 L550 V553	D242 L244 V247

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:6x74, PDBe:6x74, PDBj:6x74
• PDBsum: 6x74
• PubMed: 32999062
• UniProt: P12689|REV1_YEAST DNA repair protein REV1 (Gene Name=REV1)