Structure of PDB 6x1j Chain A

Receptor sequence

>6x1jA (length=226) Species: 1156965 (Wickerhamomyces canadensis) [Search protein sequence]

IINKKDLLGLGPNSKLIKDYKKQWTTLSKIQEETLIGNILGDVYIKKLKR
NKHFLLQFEWKNKAYIEHIVRVFDEYVISPPTLYERKNHLGNKVITWRAQ
TFEHKAFDKLGYYFMENHKKIIKPDLVLNYITERSLAYWFMDDGGKWDYN
KKTKNKSLVLHTQGFKKEEVEILINDLNIKFNLNCSIKFNKNKPIIYIPN
KDYELFYNLVNPYIIPEMKYKLLFNV

3D structure

PDB

6x1j Structure-Function Studies of Two Yeast Homing Endonucleases that Evolved to Cleave Identical Targets with Dissimilar Rates and Specificities.

Chain

Resolution

1.945 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	G16 N18 S19 K20 D47 Y49 K51 Q62 E64 K66 N93 L95 W152 D153 K196	G11 N13 S14 K15 D42 Y44 K46 Q57 E59 K61 N88 L90 W147 D148 K191
BS02	dna	A	P17 N18 K23 K26 I83 S84 R91 R103 F107 E108 D148 Y154 H166 Q168 G169 N195	P12 N13 K18 K21 I78 S79 R86 R98 F102 E103 D143 Y149 H161 Q163 G164 N190

Gene Ontology

	Molecular Function
GO:0004519	endonuclease activity

	Biological Process
GO:0000373	Group II intron splicing
GO:0006314	intron homing
GO:0045292	mRNA cis splicing, via spliceosome

	Cellular Component
GO:0005739	mitochondrion

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:6x1j, PDBe:6x1j, PDBj:6x1j
PDBsum	6x1j
PubMed	35317996
UniProt	Q34807\|IEND1_WICCA Probable intron-encoded endonuclease 1

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