Structure of PDB 6wo1 Chain A

Receptor sequence

>6wo1A (length=551) Species: 5207 (Cryptococcus neoformans)

GLDYSFIGLSGGQIFQEMMLRHDVKQVFGYPGGAILPVFDAIYNSPHFEF
VLPRHEQGAGHMAEGYARVSGKPGVVLVTSGPGATNVITPMQDALSDGVP
MVVFCGQVATNLIGSDAFQEADVVGISRSCTKWNVMVKDIAELPRRINEA
FKIATTGRPGPVLVDLPKDVTAAILRTPIPAPLPGDADLITEAAQMINKA
KRPIIFAGNGVLSSPEGPKLLKELSDKGRIPVTTTLQGLGAFDERDEKSL
HMIGMHGSAYANFAMQEADVLIALGVRFDDRVTGKVDTFAPAAKAAAAEG
RGGIIHFEIQPKNINKIVEGQIPVLGDVVASLGELVPQIEAVDRSAWIGR
CKATKERYPFTYTPSQEGQKLKPQEVVQELDRQAEALGKEKFVISTGVGQ
HQMWACQYYRWTEPRSWVSSGGLGTMGFGLPSAIGAKVAAPEKYVIDIDG
DASFSMTAMELATASQYDIGVKVLLFNNETNPDFVKLSESMGAKGLRCTK
LEDLPRMMKEFLEYDGKRPIVLECLVSSEHVYPMIPAGKALHEQLLHPLL
R

3D structure

• PDB: 6wo1 Structures of fungal and plant acetohydroxyacid synthases.
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y147 G149 G150 A151 I152 E173 T196 F235 Q236 E237 K285 M396 V423 V539 G565 M567 D592 N619
• Catalytic site (residue number reindexed from 1): Y30 G32 G33 A34 I35 E56 T79 F118 Q119 E120 K168 M255 V282 V398 G424 M426 D451 N478
• Enzyme Commision number: 2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	D214 R275 G349 G351 S354 T376 L377 Q378 I394 G395 M396 G416 V417 R418 D420 R422 V423 E449 I450 D468 V469 Q543 G562 G563	D97 R158 G208 G210 S213 T235 L236 Q237 I253 G254 M255 G275 V276 R277 D279 R281 V282 E308 I309 D327 V328 Q402 G421 G422
BS02	8GF	A	G565 T566 M567	G424 T425 M426
BS03	DPO	A	V539 G540 Q541 H542 G591 D592 A593 S594	V398 G399 Q400 H401 G450 D451 A452 S453
BS04	MG	A	D592 N619	D451 N478

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0003984 acetolactate synthase activity
• GO:0016740 transferase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0046872 metal ion binding
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0008652 amino acid biosynthetic process
• GO:0009082 branched-chain amino acid biosynthetic process
• GO:0009097 isoleucine biosynthetic process
• GO:0009099 L-valine biosynthetic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005948 acetolactate synthase complex

External links

• PDB: RCSB:6wo1, PDBe:6wo1, PDBj:6wo1
• PDBsum: 6wo1
• PubMed: 32640464
• UniProt: Q96VZ6