Structure of PDB 6wbr Chain A

Receptor sequence

>6wbrA (length=942) Species: 351607 (Acidothermus cellulolyticus 11B) [Search protein sequence]

GTVPVTWRLGVDVGERSIGLAAVSYEEDKPKEILAAVSWIHDGGVGDERS
GASRLALRGMARRARRLRRFRRARLRDLDMLLSELGWTPLPDKNVSPVDA
WLARKRLAEEYVVDETERRRLLGYAVSHMARHRGWRNPWTTIKDLKNLPQ
PSDSWERTRESLEARYSVSLEPGTVGQWAGYLLQRAPGIRLNPTQQSLSN
ATAFETRLRQEDVLWELRCIADVQGLPEDVVSNVIDAVFCQKRPSVPAER
IGRDPLDPSQLRASRACLEFQEYRIVAAVANLRIRDGSGSRPLSLEERNA
VIEALLAQTERSLTWSDIALEILKLPNESDLTSVPEEDGPSSLAYSQFAP
FDETSARIAEFIAKNRRKIPTFAQWWQEQDRTSRSDLVAALADNSIAGLL
VHLPDAELEALEGLALPSGRVAYSRLTLSGLTRVMRDDGVDVHNARKTCF
GVDDNWRPPLPALHEATGHPVVDRNLAILRKFLSSATMRWGPPQSIVVEL
GSAGGYAAVALRDRLLSYGEKNGVAQVAVFRGGVTAEARRWLDISIERLF
SRVAIFAQSTSTKRLDRRHHAVDAVVLTTLTPGVAKTLADARSRRVSAST
EEPQSPAYRQWKESCSGLGDLLISTAARDSIAVAAPLRLRPTGALHEETL
RAFSEHTVGAAWKGAELRRIVEPEVYAAFLALTDPGGRFLKVSPSEDVLP
ADENRHIVLSDRVLGPRDRVKLFPDDRGSIRVRGGAAYIASFHHARVFRW
GSSHSPSFALLRVSLADLAVAGLLRDGVDVFTAELPPWTPAWRYASIALV
KAVESGDAKQVGWLVPGDELDFGPEGVTTAAGDLSMFLKYFPERHWVVTG
FEDDKRINLKPAFLSAEQAEVLRTERSDRPDTLTEAGEILAQFFPRCWRA
TVAKVLCHPGLTVIRRTALGQPRWRRGHLPYSWRPWSADPWS

3D structure

PDB

6wbr The molecular basis for recognition of 5'-NNNCC-3' PAM and its methylation state by Acidothermus cellulolyticus Cas9.

Chain

Resolution

2.91 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	H47 D48 S59 R60 L61 R64 R68 R69 R71 R72 L73 R74 R78 L96 D98 N100 V101 S102 P103 W107 H134 R137 H138 R139 G140 W141 P144 G194 I195 R196 L197 N198 T200 N212 Q253 K254 P256 S257 R262 R323 Q359 H481 H486 P487 R491 R497 K498 S501 S502 M505 P828 L829 R830 L831 R832 P833 T834 G835 A836 L837 H838 L842 A844 R925 G969 V970 D971 F973 T1109 L1111 Q1113 R1115 W1116 H1120 P1122	H41 D42 S53 R54 L55 R58 R62 R63 R65 R66 L67 R68 R72 L90 D92 N94 V95 S96 P97 W101 H128 R131 H132 R133 G134 W135 P138 G188 I189 R190 L191 N192 T194 N200 Q241 K242 P244 S245 R250 R311 Q347 H464 H469 P470 R474 R480 K481 S484 S485 M488 P636 L637 R638 L639 R640 P641 T642 G643 A644 L645 H646 L650 A652 R733 G777 V778 D779 F781 T917 L919 Q921 R923 W924 H928 P930
BS02	dna	A	R55 W141 W145 R219 G264 R274 A356 Y357 S358 I408 R437 Y686 E839 E840 T841 D1025 K1047 R1088 R1091 T1093 K1096	R49 W135 W139 R207 G252 R262 A344 Y345 S346 I396 R420 Y506 E647 E648 T649 D833 K855 R896 R899 T901 K904
BS03	dna	A	R919 F934 G1042 E1044	R727 F742 G850 E852

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding

View graph for
Molecular Function

External links

PDB	RCSB:6wbr, PDBe:6wbr, PDBj:6wbr
PDBsum	6wbr
PubMed	33311465
UniProt	A0LWB3

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