Structure of PDB 6w8v Chain A

Receptor sequence

>6w8vA (length=831) Species: 10090 (Mus musculus) [Search protein sequence]

DRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLAR
VTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYI
HSKVKVIYKAPSENWAMEGGEDGKTYFFQLWYNQEYARFESPPKTQPTED
NKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGD
SVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDA
PEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTD
INMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEA
YNSKTKNFEDPPNHARSPGNKLPKLRTLDVFSGCGGLSEGFHQAGISETL
WAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQK
GDVEMLCGGPPCQGFSGMNRFNSYSKFKNSLVVSFLSYCDYYRPRFFLLE
NVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIIL
AAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTIT
VRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKD
MSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGY
SSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRL
EWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFG
NILDRHRQVGNAVPPPLAKAIGLEIKLCLLS

3D structure

PDB

6w8v DNMT1 activity, base flipping mechanism and genome-wide DNA methylation are regulated by the DNA sequence context

Chain

Resolution

3.12 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	C1229 E1269 R1313 R1315
Catalytic site (residue number reindexed from 1)	C462 E500 R544 R546
Enzyme Commision number	2.1.1.37: DNA (cytosine-5-)-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	G1234 R1237 R1272 S1276 Q1343 R1427 R1492 C1501 H1504 T1505 R1508 H1509 W1512 M1535	G467 R470 R503 S507 Q574 R658 R723 C732 H735 T736 R739 H740 W743 M766
BS02	dna	A	S981 Y983 K985 G1226 P1227 P1228 C1229 Q1230 S1233 M1235 F1238 E1269 V1271 T1312 R1313 R1314 R1315 N1510 T1527 T1530 K1537 N1580	S240 Y242 K244 G459 P460 P461 C462 Q463 S466 M468 F471 E500 V502 T543 R544 R545 R546 N741 T758 T761 K768 N811
BS03	SAH	A	F1148 S1149 G1150 L1154 E1171 M1172 D1193 C1194 N1580 A1581 V1582	F381 S382 G383 L387 E404 M405 D426 C427 N811 A812 V813
BS04	ZN	A	C1479 C1481 C1487 H1504	C710 C712 C718 H735
BS05	ZN	A	H796 C823 C897	H65 C92 C156

Gene Ontology

	Molecular Function
GO:0003682	chromatin binding
GO:0008168	methyltransferase activity

View graph for
Molecular Function

External links

PDB	RCSB:6w8v, PDBe:6w8v, PDBj:6w8v
PDBsum	6w8v
PubMed
UniProt	P13864\|DNMT1_MOUSE DNA (cytosine-5)-methyltransferase 1 (Gene Name=Dnmt1)

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