Structure of PDB 6v62 Chain A

Receptor sequence

>6v62A (length=484) Species: 9606 (Homo sapiens) [Search protein sequence]

VSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSV
TFDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEEL
FLWVPRKLLMTVESAKNSVLGPLYSQDRILQAMGNIALAFHLLCERASPN
SFWQPYIQTLPSEYDTPLYFEEDEVRYLQSTQAIHDVFSQYKNTARQYAY
FYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQFQIPTEDGSRVTLA
LIPLADMCNHTNGLITTGYNLEDDRCECVALQDFRAGEQIYIFYGTRSNA
EFVIHSGFFFDNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFA
LHFTEPPISAQLLAFLRVFCMTEEELKEHLLGDSAIDRIFTLGNSEFPVS
WDNEVKLWTFLEDRASLLLKTYKTTIEEDKSVLKNHDLSVRAKMAIKLRL
GEKEILEKAVKSAAVNREYYRQQMEEKAPLPKYE

3D structure

PDB

6v62 An engineered variant of SETD3 methyltransferase alters target specificity from histidine to lysine methylation.

Chain

Resolution

2.36 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.1.1.85: protein-histidine N-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	M151 N153 I154 N211 R214 Q215 Q254 F255 Q256 D274 I283 T285 G286 Y287 Y312 R315	M133 N135 I136 N193 R196 Q197 Q236 F237 Q238 D256 I265 T267 G268 Y269 Y294 R297
BS02	SAH	A	R74 E103 F105 R253 D274 M275 N277 H278 Y312 S324 F326	R56 E85 F87 R235 D256 M257 N259 H260 Y294 S306 F308

Gene Ontology

	Molecular Function
GO:0003713	transcription coactivator activity
GO:0003779	actin binding
GO:0005515	protein binding
GO:0008168	methyltransferase activity
GO:0008170	N-methyltransferase activity
GO:0008276	protein methyltransferase activity
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
GO:0016279	protein-lysine N-methyltransferase activity
GO:0018064	protein-L-histidine N-tele-methyltransferase activity
GO:0042800	histone H3K4 methyltransferase activity
GO:0046975	histone H3K36 methyltransferase activity
GO:0061629	RNA polymerase II-specific DNA-binding transcription factor binding

	Biological Process
GO:0006338	chromatin remodeling
GO:0018021	peptidyl-histidine methylation
GO:0018023	peptidyl-lysine trimethylation
GO:0030047	actin modification
GO:0032259	methylation
GO:0045893	positive regulation of DNA-templated transcription
GO:0045944	positive regulation of transcription by RNA polymerase II
GO:0051149	positive regulation of muscle cell differentiation
GO:0070472	regulation of uterine smooth muscle contraction

	Cellular Component
GO:0000785	chromatin
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:6v62, PDBe:6v62, PDBj:6v62
PDBsum	6v62
PubMed	31911441
UniProt	Q86TU7\|SETD3_HUMAN Actin-histidine N-methyltransferase (Gene Name=SETD3)

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