Structure of PDB 6uy0 Chain A

Receptor sequence

>6uy0A (length=1210) Species: 9913 (Bos taurus)

NDPNPCPESSASFLSRITFWWITGMMVQGYRQPLESTDLWSLNKEDTSEQ
VVPVLVKNWKKECAKSPSLFKVLYKTFGPYFLMSFLFKAVHDLMMFAGPE
ILKLLINFVNDKKAPEWQGYFYTALLFISACLQTLVLHQYFHICFVSGMR
IKTAVIGAVYRKALVITNAARKSSTVGEIVNLMSVDAQRFMDLATYINMI
WSAPLQVILALYLLWLNLGPSVLAGVAVMVLMVPLNAVMAMKTKTYQVAH
MKSKDNRIKLMNEILNGIKVLKLYAWELAFKDKVLAIRQEELKVLKKSAY
LAAVGTFTWVCTPFLVALSTFAVYVTVDENNILDAQKAFVSLALFNILRF
PLNILPMVISSIVQASVSLKRLRVFLSHEDLDPDSIQRRPITNSITVKNA
TFTWARNDPPTLHGITFSVPEGSLVAVVGQVGCGKSSLLSALLAEMDKVE
GHVTVKGSVAYVPQQAWIQNISLRENILFGRQLQERYYKAVVEACALLPD
LEILPSGDRTEIGEKGVNLSGGQKQRVSLARAVYCDSDVYLLDDPLSAVD
AHVGKHIFENVIGPKGLLKNKTRLLVTHAISYLPQMDVIIVMSGGKISEM
GSYQELLARDGAFAEFLRTYAETWKLVEADKAQTGQVKLSVYWDYMKAIG
LFISFLSIFLFLCNHVASLVSNYWLSLWTDDPIVNGTQEHTQVRLSVYGA
LGISQGITVFGYSMAVSIGGIFASRRLHLDLLHNVLRSPISFFERTPSGN
LVNRFSKELDTVDSMIPQVIKMFMGSLFNVIGACIIILLATPMAAVIIPP
LGLIYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQ
ERFIRQSDLKVDENQKAYYPSIVANRWLAVRLECVGNCIVLFASLFAVIS
RHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSEMETNIVAVERLKEYSETE
KEAPWQIQDMAPPKDWPQVGRVEFRDYGLRYREDLDLVLKHINVTIDGGE
KVGIVGRTGAGKSSLTLGLFRIKESAEGEIIIDDINIAKIGLHDLRFKIT
IIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKGFVSALPDKLNH
ECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQST
IRTQFDDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEWGSPSDLLQQRGLF
YSMAKDSGLV

3D structure

• PDB: 6uy0 Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses.
• Chain: A
• Resolution: 3.23 Å

Enzymatic activity

• Enzyme Commision number: 7.6.2.2: ABC-type xenobiotic transporter.
  7.6.2.3: ABC-type glutathione-S-conjugate transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	W653 G681 G683 K684 S685 S686 Q713 E1427 N1428 S1430 G1432 Q1433	W404 G432 G434 K435 S436 S437 Q464 E1107 N1108 S1110 G1112 Q1113
BS02	ADP	A	N767 S769 E1064 Y1301 G1329 G1331 K1332 S1333 S1334	N518 S520 E744 Y981 G1009 G1011 K1012 S1013 S1014
BS03	MG	A	S685 Q713	S436 Q464
BS04	MG	A	S1333 Q1374	S1013 Q1054
BS05	CLR	A	Y962 M966 F1093 R1130 S1250 M1253	Y642 M646 F773 R810 S930 M933
BS06	CLR	A	K488 I603 I606	K244 I359 I362

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008559 ABC-type xenobiotic transporter activity
• GO:0015431 ABC-type glutathione S-conjugate transporter activity
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0022857 transmembrane transporter activity
• GO:0034634 glutathione transmembrane transporter activity
• GO:0042910 xenobiotic transmembrane transporter activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0006869 lipid transport
• GO:0009410 response to xenobiotic stimulus
• GO:0034775 glutathione transmembrane transport
• GO:0042908 xenobiotic transport
• GO:0050729 positive regulation of inflammatory response
• GO:0055085 transmembrane transport
• GO:0070729 cyclic nucleotide transport
• GO:0071716 leukotriene transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0016323 basolateral plasma membrane

External links

• PDB: RCSB:6uy0, PDBe:6uy0, PDBj:6uy0
• PDBsum: 6uy0
• PubMed: 32458799
• UniProt: Q8HXQ5|MRP1_BOVIN Multidrug resistance-associated protein 1 (Gene Name=ABCC1)