Structure of PDB 6tsi Chain A

Receptor sequence

>6tsiA (length=540) Species: 208964 (Pseudomonas aeruginosa PAO1)

MKAAEQYQGAASAVDPAHVVRTNGAPDMSESEFNEAKQIYFQRCAGCHGV
LRKGATGKPLTPDITQQRGQQYLEALITYGTPLGMPNWGSSGELSKEQIT
LMAKYIQHTPPQPPEWGMPEMRESWKVLVKPEDRPKKQLNDLDLPNLFSV
TLRDAGQIALVDGDSKKIVKVIDTGYAVHISRMSASGRYLLVIGRDARID
MIDLWAKEPTKVAEIKIGIEARSVESSKFKGYEDRYTIAGAYWPPQFAIM
DGETLEPKQIVSTRGMTVDTQTYHPEPRVAAIIASHEHPEFIVNVKETGK
VLLVNYKDIDNLTVTSIGAAPFLHDGGWDSSHRYFMTAANNSNKVAVIDS
KDRRLSALVDVGKTPHPGRGANFVHPKYGPVWSTSHLGDGSISLIGTDPK
NHPQYAWKKVAELQGQGGGSLFIKTHPKSSHLYVDTTFNPDARISQSVAV
FDLKNLDAKYQVLPIAEWADLGEGAKRVVQPEYNKRGDEVWFSVWNGKND
SSALVVVDDKTLKLKAVVKDPRLITPTGKFNVYNTQHDVY

3D structure

• PDB: 6tsi Structure of heme d 1 -free cd 1 nitrite reductase NirS.
• Chain: A
• Resolution: 2.38 Å

Enzymatic activity

• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).
  1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	A	C47 C50 H51 T59 K61 L63 R71 I80 T84 L86 G87 M88 P89 W91	C44 C47 H48 T56 K58 L60 R68 I77 T81 L83 G84 M85 P86 W88
BS02	DHE	A	R156 H182 I183 R198 R225 S226 Y245 A283 A284 H327 R372 F425 W498	R153 H179 I180 R195 R222 S223 Y242 A280 A281 H324 R369 F422 W495

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0050418 hydroxylamine reductase activity
• GO:0050421 nitrite reductase (NO-forming) activity

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:6tsi, PDBe:6tsi, PDBj:6tsi
• PDBsum: 6tsi
• PubMed: 32510465
• UniProt: P24474|NIRS_PSEAE Nitrite reductase (Gene Name=nirS)