Structure of PDB 6tnt Chain A

Receptor sequence

>6tntA (length=1583) Species: 9606 (Homo sapiens)

MIAARDLQEFVPFGRDHCKHHPNAKESWQLRKGVSEIGEDVDYDEELYVA
GNMVIWSKGSKSQALAVYKAFTVDSPVQQALWCDFIISQDKSEKAYSSNE
VEKCICILQSSCINMHSIEGKDYIASLPFQVANVWPTKYGLLFERSAPLP
TMFSMLHPLDEITPLVCKSRVQYVVDHAMKIVFLNTDPSIVMTYDAVQNV
HSVWTLRRVKSEEENVSFSGVQRFNISSHNIVPELCIDHLWTETEKNSQA
SKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNIPAK
DAAPVEKIDTMLVLEGSGNLVLYTGVVRVGKVFIPGLPQLGTYIHSIRDP
VHNRVTLELSNGSMVRITIPEIATSELVQTCLQAIKFILPKEIAVQMLVK
WYNVHSAPGGPSYHSEWNLFVTCLMNMMGYNTDRLAWTDDDWEYLLNSDY
HQNVESHLLNRSLCLSLSLDSSTLLFTHIPAIFFVLHLVYEELKLNTLMG
EGICSLVELLVQLARDLKLGPYVDHYYRDYPTLVRTTGQVCTIDPGQTGF
MHHPSFFTSEPPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALY
ILGDESLSSQYLTRITVSSLAERLVVWMTNVGFTLRDLETLPFGIALPIR
DAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPEEDDGMNDMNHEV
MSLIWSEDLRVQDVRRLLQSAHPVRVNVVQYPELSDHEFIEEKENRLLQL
CQRTMALPVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLNSG
NIDVPPNMTSWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYA
GFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMS
ITRLLSIHIPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVL
LAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPE
QLYQYMVGGHSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADW
LRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRE
NSILNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKDFMTY
LSAPNASVTGPHNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGG
EMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTD
NRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEQTK
EELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGTQHLKSILSKDGVLY
VKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALL
SFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYIAMDQAIR
RLGRREMSETSELWQIKLVLEFFSSRSHQERLQNHPKRGLFMNSEFLPVV
KCTIDNTLDQWLQVGGDMCVHAYLSGQPLEESQLSMLACFLVYHSVPAPQ
HLPPSTSFAELLFKFKQLKMPVRALLRLAPLLL

3D structure

• PDB: 6tnt Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation.
• Chain: A
• Resolution: 3.78 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	E1030 E1480 N1481 W1643 E1649	E707 E1132 N1133 W1295 E1301

Gene Ontology

Molecular Function

• GO:0060090 molecular adaptor activity

Biological Process

• GO:0007091 metaphase/anaphase transition of mitotic cell cycle
• GO:0007346 regulation of mitotic cell cycle
• GO:0016567 protein ubiquitination
• GO:0031145 anaphase-promoting complex-dependent catabolic process
• GO:0051301 cell division
• GO:0051445 regulation of meiotic cell cycle
• GO:0070936 protein K48-linked ubiquitination
• GO:0070979 protein K11-linked ubiquitination
• GO:0141198 protein branched polyubiquitination

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005680 anaphase-promoting complex
• GO:0005829 cytosol

External links

• PDB: RCSB:6tnt, PDBe:6tnt, PDBj:6tnt
• PDBsum: 6tnt
• PubMed: 33789095
• UniProt: Q9H1A4|APC1_HUMAN Anaphase-promoting complex subunit 1 (Gene Name=ANAPC1)