Structure of PDB 6t1u Chain A

Receptor sequence

>6t1uA (length=635) Species: 5482 (Candida tropicalis)

SRDVLSTLKKNNKNTLLLFGSQTGTAEDYANKLSRELHSRFGLKTMVADF
ADYDWDNFGDITEDILVFFIVATYGEGEPTDNADEFHTWLTEEADTLSTL
RYTVFGLGNSTYEFFNAIGRKFDRLLSEKGGDRFAEYAEGDDGTGTLDED
FMAWKDNVFDALKNDLNFEEKELKYEPNVKLTERDDLSAADSQVSLGEPN
KKYINSEGIDLTKGPFDHTHPYLARITETRELFSSKERHCIHVEFDISES
NLKYTTGDHLAIWPSNSDENIKQFAKCFGLEDKLDTVIELKALDSTYTIP
FPTPITYGAVIRHHLEISGPVSRQFFLSIAGFAPDEETKKTFTRLGGDKQ
EFATKVTRRKFNIADALLYSSNNTPWSDVPFEFLIENIQHLTPRYYSISS
SSLSEKQLINVTAVVEAEEEADGRPVTGVVTNLLKNIEIAQNKTGEKPLV
HYDLSGPRGKFNKFKLPVHVRRSNFKLPKNSTTPVILIGPGTGVAPLRGF
VRERVQQVKNGVNVGKTLLFYGCRNSNEDFLYKQEWAEYASVLGENFEMF
NAFSRQDPSKKVYVQDKILENSQLVHELLTEGAIIYVCGDASRMARDVQT
TISKIVAKSREISEDKAAELVKSWKVQNRYQEDVW

3D structure

• PDB: 6t1u Biochemical and structural insights into the cytochrome P450 reductase from Candida tropicalis.
• Chain: A
• Resolution: 1.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S441 C632 D677 W679
• Catalytic site (residue number reindexed from 1): S397 C588 D633 W635
• Enzyme Commision number: 1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	Y118 G363 P364 K404 F405 R438 Y439 Y440 S441 T456 A457 E460 V470 T471 G472 V473 V474 T475 W679	Y74 G319 P320 K360 F361 R394 Y395 Y396 S397 T412 A413 E416 V426 T427 G428 V429 V430 T431 W635
BS02	FMN	A	S65 Q66 T67 T69 A70 T117 Y118 G119 G121 L151 G152 N153 Y156 F159 N160 D186	S21 Q22 T23 T25 A26 T73 Y74 G75 G77 L107 G108 N109 Y112 F115 N116 D142

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003958 NADPH-hemoprotein reductase activity
• GO:0003959 NADPH dehydrogenase activity
• GO:0009055 electron transfer activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding

Biological Process

• GO:0006696 ergosterol biosynthetic process
• GO:0016126 sterol biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005741 mitochondrial outer membrane
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005829 cytosol
• GO:0005886 plasma membrane

External links

• PDB: RCSB:6t1u, PDBe:6t1u, PDBj:6t1u
• PDBsum: 6t1u
• PubMed: 31882753
• UniProt: Q5PXH3