Structure of PDB 6t0l Chain A

Receptor sequence
>6t0lA (length=422) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]
IATRVNGTPPPEVPIADIELGSLDFWALDDDVRDGAFATLRREAPISFWP
TIELPGFVTGNGHWALTKYDDVFYASRHPDIFSSYPNITINDQTPELAEY
FGSMIVLDDPRHQRLRSIVSRAFTPKVVARIEAAVRDRAHRLVSSMIANN
PDRQADLVSELAGPLPLQIICDMMGIPKADHQRIFHWTNVILGFGDPDLA
TDFDEFMQVSADIGAYATALAEDRRVNHHDDLTSSLVEAEVDGERLSSRE
IASFFILLVVAGNETTRNAITHGVLALSRYPEQRDRWWSDFDGLAPTAVE
EIVRWASPVVYMRRTLTQDIELRGTKMAAGDKVSLWYCSANRDESKFADP
WTFDLARNPNPHLGFGGGGAHFCLGANLARREIRVAFDELRRQMPDVVAT
EEPARLLSQFIHGIKTLPVTWS
3D structure
PDB6t0l Metabolic Fate of Human Immunoactive Sterols in Mycobacterium tuberculosis.
ChainA
Resolution1.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G199 E270 T271 C379 L380 G381
Catalytic site (residue number reindexed from 1) G193 E264 T265 C373 L374 G375
Enzyme Commision number 1.14.15.14: methyl-branched lipid omega-hydroxylase.
1.14.15.28: cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming].
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A I111 H118 R122 A267 G268 T271 P314 R320 G370 F371 H377 C379 L380 G381 A385 I105 H112 R116 A261 G262 T265 P308 R314 G364 F365 H371 C373 L374 G375 A379
BS02 M8N A L263 V266 A267 T271 F416 L257 V260 A261 T265 F410
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008395 steroid hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0031073 cholesterol 26-hydroxylase activity
GO:0036199 cholest-4-en-3-one 26-monooxygenase activity
GO:0046872 metal ion binding
GO:0070402 NADPH binding
Biological Process
GO:0006631 fatty acid metabolic process
GO:0006707 cholesterol catabolic process
GO:0010430 fatty acid omega-oxidation
GO:0097089 methyl-branched fatty acid metabolic process

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6t0l, PDBe:6t0l, PDBj:6t0l
PDBsum6t0l
PubMed33359098
UniProtP9WPP3|CP124_MYCTU Methyl-branched lipid omega-hydroxylase (Gene Name=cyp124)

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