Structure of PDB 6t0k Chain A

Receptor sequence
>6t0kA (length=430) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]
HHMGLNTAIATRVNGTPPPEVPIADIELGSLDFWALDDDVRDGAFATLRR
EAPISFWPTIELPGFVAGNGHWALTKYDDVFYASRHPDIFSSYPNITIND
QTPELAEYFGSMIVLDDPRHQRLRSIVSRAFTPKVVARIEAAVRDRAHRL
VSSMIANNPDRQADLVSELAGPLPLQIICDMMGIPKADHQRIFHWTNVIL
GFGDPDLATDFDEFMQVSADIGAYATALAEDRRVNHHDDLTSSLVEAEVD
GERLSSREIASFFILLVVAGNETTRNAITHGVLALSRYPEQRDRWWSDFD
GLAPTAVEEIVRWASPVVYMRRTLTQDIELRGTKMAAGDKVSLWYCSANR
DESKFADPWTFDLARNPNPHLGFGGGGAHFCLGANLARREIRVAFDELRR
QMPDVVATEEPARLLSQFIHGIKTLPVTWS
3D structure
PDB6t0k Metabolic Fate of Human Immunoactive Sterols in Mycobacterium tuberculosis.
ChainA
Resolution1.18 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G199 A267 E270 T271 T272 C379 L380 G381 E388 I417
Catalytic site (residue number reindexed from 1) G201 A269 E272 T273 T274 C381 L382 G383 E390 I419
Enzyme Commision number 1.14.15.14: methyl-branched lipid omega-hydroxylase.
1.14.15.28: cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming].
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A I111 H118 R122 L263 A267 G268 T271 P314 R320 G370 F371 H377 C379 L380 G381 I113 H120 R124 L265 A269 G270 T273 P316 R322 G372 F373 H379 C381 L382 G383
BS02 M65 A F107 I197 A267 T271 M318 F416 F109 I199 A269 T273 M320 F418
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008395 steroid hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0031073 cholesterol 26-hydroxylase activity
GO:0036199 cholest-4-en-3-one 26-monooxygenase activity
GO:0046872 metal ion binding
GO:0070402 NADPH binding
Biological Process
GO:0006631 fatty acid metabolic process
GO:0006707 cholesterol catabolic process
GO:0010430 fatty acid omega-oxidation
GO:0097089 methyl-branched fatty acid metabolic process

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6t0k, PDBe:6t0k, PDBj:6t0k
PDBsum6t0k
PubMed33359098
UniProtP9WPP3|CP124_MYCTU Methyl-branched lipid omega-hydroxylase (Gene Name=cyp124)

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