Structure of PDB 6t0g Chain A

Receptor sequence
>6t0gA (length=426) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]
LNTAIATRVNGTPPPEVPIADIELGSLDFWALDDDVRDGAFATLRREAPI
SFWPTIELPGFVAGNGHWALTKYDDVFYASRHPDIFSSYPNITINDQTPE
LAEYFGSMIVLDDPRHQRLRSIVSRAFTPKVVARIEAAVRDRAHRLVSSM
IANNPDRQADLVSELAGPLPLQIICDMMGIPKADHQRIFHWTNVILGFGD
PDLATDFDEFMQVSADIGAYATALAEDRRVNHHDDLTSSLVEAEVDGERL
SSREIASFFILLVVAGNETTRNAITHGVLALSRYPEQRDRWWSDFDGLAP
TAVEEIVRWASPVVYMRRTLTQDIELRGTKMAAGDKVSLWYCSANRDESK
FADPWTFDLARNPNPHLGFGGGGAHFCLGANLARREIRVAFDELRRQMPD
VVATEEPARLLSQFIHGIKTLPVTWS
3D structure
PDB6t0g Metabolic Fate of Human Immunoactive Sterols in Mycobacterium tuberculosis.
ChainA
Resolution1.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G199 A267 E270 T271 T272 C379 L380 G381 E388 I417
Catalytic site (residue number reindexed from 1) G197 A265 E268 T269 T270 C377 L378 G379 E386 I415
Enzyme Commision number 1.14.15.14: methyl-branched lipid omega-hydroxylase.
1.14.15.28: cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming].
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A I111 H118 R122 A267 G268 T271 T272 P314 R320 G370 F371 H377 C379 L380 G381 A385 I109 H116 R120 A265 G266 T269 T270 P312 R318 G368 F369 H375 C377 L378 G379 A383
BS02 VD3 A N93 T95 N91 T93
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008395 steroid hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0031073 cholesterol 26-hydroxylase activity
GO:0036199 cholest-4-en-3-one 26-monooxygenase activity
GO:0046872 metal ion binding
GO:0070402 NADPH binding
Biological Process
GO:0006631 fatty acid metabolic process
GO:0006707 cholesterol catabolic process
GO:0010430 fatty acid omega-oxidation
GO:0097089 methyl-branched fatty acid metabolic process

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6t0g, PDBe:6t0g, PDBj:6t0g
PDBsum6t0g
PubMed33359098
UniProtP9WPP3|CP124_MYCTU Methyl-branched lipid omega-hydroxylase (Gene Name=cyp124)

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