Structure of PDB 6spn Chain A

Receptor sequence
>6spnA (length=544) Species: 562 (Escherichia coli) [Search protein sequence]
AKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGHEVNFICADDA
HGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEENR
QLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQ
YGDNCEVCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQA
WTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVW
LDAPIGYMGSFKNLCDKRGDSVSFDEYWKKDSTAELYHFIGKDIVYFHSL
FWPAMLEGSNFRKPSNLFVHGYVTVNGAKMSKSRGTFIKASTWLNHFDAD
SLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKR
FDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALADLANR
YVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAE
AFLNTELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASK
3D structure
PDB6spn Use of beta3-methionine as an amino acid substrate of Escherichia coli methionyl-tRNA synthetase.
ChainA
Resolution1.45 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.10: methionine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A C145 C148 C158 C161 C142 C145 C155 C158
BS02 B3M A A12 L13 D52 W253 A256 P257 Y260 A9 L10 D49 W250 A253 P254 Y257 MOAD: Kd=1.2mM
BS03 B3M A K217 K295 D296 Y325 I367 K214 K292 D293 Y322 I364 MOAD: Kd=1.2mM
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004825 methionine-tRNA ligase activity
GO:0005524 ATP binding
Biological Process
GO:0006418 tRNA aminoacylation for protein translation
GO:0006431 methionyl-tRNA aminoacylation

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Molecular Function
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Biological Process
External links
PDB RCSB:6spn, PDBe:6spn, PDBj:6spn
PDBsum6spn
PubMed31862305
UniProtP00959|SYM_ECOLI Methionine--tRNA ligase (Gene Name=metG)

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