Structure of PDB 6s6s Chain A

Receptor sequence

>6s6sA (length=1468) Species: 192 (Azospirillum brasilense)

CGVGFIAAIDGKPRRSVVEKGIEALKAVWHRGAVDADGKTGDGAGIHVAV
PQKFFKDHVKVIGHRAPDNKLAVGQVFLPRISLDAQEACRCIVETEILAF
GYYIYGWRQVPINVDIIGEKANATRPEIEQIIVGNNKGVSDEQFELDLYI
IRRRIEKAVKGEQINDFYICSLSARSIIYKGMFLAEQLTTFYPDLLDERF
ESDFAIYHQRYSTNTFPTWPLAQPFRMLAHNGEINTVKGNVNWMKAHETR
MEHPAFGTHMQDLKPVIGVGLSDSGSLDTVFEVMVRAGRTAPMVKMMLVP
QALTSSQTTPDNHKALIQYCNSVMEPWDGPAALAMTDGRWVVGGMDRNGL
RPMRYTITTDGLIIGGSETGMVKIDETQVIEKGRLGPGEMIAVDLQSGKL
YRDRELKDHLATLKPWDKWVQNTTHLDELVKTASLKDMDKAELRRRQQAF
GLTMEDMELILHPMVEDGKEAIGSMGDDSPIAVLSDKYRGLHHFFRQNFS
QVTNPPIDSLRERRVMSLKTRLGNLGNILDEDETQTRLLQLESPVLTTAE
FRAMRDYMGDTAAEIDATFPVDGGPEALRDALRRIRQETEDAVRGGATHV
ILTDEAMGPARAAIPAILATGAVHTHLIRSNLRTFTSLNVRTAEGLDTHY
FAVLIGVGATTVNAYLAQEAIAERHRRGLFGSMPLEKGMANYKKAIDDGL
LKIMSKMGISVISSYRGGGNFEAIGLSRALVAEHFPAMVSRISGIGLNGI
QKKVLEQHATAYNEEVVALPVGGFYRFRKSGDRHGWEGGVIHTLQQAVTN
DSYTTFKKYSEQVNKRPPMQLRDLLELRSTKAPVPVDEVESITAIRKRFI
TPGMSMGALSPEAHGTLNVAMNRIGAKSDSGEGGEDPARFRPDKNGDNWN
SAIKQVASGRFGVTAEYLNQCRELEIKVAQGAKPGEGGQLPGFKVTEMIA
RLRHSTPGVMLISPPPHHDIYSIEDLAQLIYDLKQINPDAKVTVKLVSRS
GIGTIAAGVAKANADIILISGNSGGTGASPQTSIKFAGLPWEMGLSEVHQ
VLTLNRLRHRVRLRTDGGLKTGRDIVIAAMLGAEEFGIGTASLIAMGCIM
VRQCHSNTCPVGVCVQDDKLRQKFVGTPEKVVNLFTFLAEEVREILAGLG
FRSLNEVIGRTDLLHQVSRGAEHLDDLDLNPRLAQVDPGENARYCTLQGR
NEVPDTLDARIVADARPLFEEGEKMQLAYNARNTQRAIGTRLSSMVTRKF
GMFGLQPGHITIRLRGTAGQSLGAFAVQGIKLEVMGDANDYVGKGLSGGT
IVVRPTTSSPLETNKNTIIGNTVLYGATAGKLFAAGQAGERFAVRNSGAT
VVVEGCGSNGCEYMTGGTAVILGRVGDNFAAGMTGGMAYVYDLDDSLPLY
INDESVIFQRIEVGHYESQLKHLIEEHVTETQSRFAAEILNDWAREVTKF
WQVVPKEMLNRLEVPVHL

3D structure

• PDB: 6s6s Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.
• Chain: A
• Resolution: 3.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C1 R31 Y211 N231 G232 M479 E886 Q934 K937 Q943
• Catalytic site (residue number reindexed from 1): C1 R31 Y211 N231 G232 M475 E882 Q930 K933 Q939
• Enzyme Commision number: 1.4.1.13: glutamate synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	A	P856 G857 M858 S859 E886 Q909 K931 Q934 G1029 T1030 D1070 G1071 G1072 G1093 T1094	P852 G853 M854 S855 E882 Q905 K927 Q930 G1025 T1026 D1066 G1067 G1068 G1089 T1090
BS02	F3S	A	M479 C1102 I1103 M1104 V1105 R1106 C1108 C1113 V1115 C1118	M475 C1098 I1099 M1100 V1101 R1102 C1104 C1109 V1111 C1114

Gene Ontology

Molecular Function

• GO:0004355 glutamate synthase (NADPH) activity
• GO:0015930 glutamate synthase activity
• GO:0016491 oxidoreductase activity
• GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors
• GO:0046872 metal ion binding
• GO:0051538 3 iron, 4 sulfur cluster binding

Biological Process

• GO:0006537 glutamate biosynthetic process
• GO:0006541 glutamine metabolic process
• GO:0019676 ammonia assimilation cycle
• GO:0097054 L-glutamate biosynthetic process

External links

• PDB: RCSB:6s6s, PDBe:6s6s, PDBj:6s6s
• PDBsum: 6s6s
• PubMed: 31473159
• UniProt: Q05755|GLTB_AZOBR Glutamate synthase [NADPH] large chain (Gene Name=gltB)