Structure of PDB 6rku Chain A

Receptor sequence
>6rkuA (length=511) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
ITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMNVLGN
DWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQG
NFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPD
VMPTKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEG
LMEHIPGPDFPTAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRE
TIIVHEIPYQVNKARLIEKIAELVKEKRVEGISALRDESDKDGMRIVIEV
KRDAVGEVVLNNLYSQTQLQVSFGINMVALHHGQPKIMNLKDIIAAFVRH
RREVVTRRTIFELRKARDRAHILEALAVALANIDPIIELIRHAPTPAEAK
TALVANPWQLGNVAAAGDDAARPEWLEPEFGVGLYYLTEQQAQAILDLRL
QKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELELVREQF
GDKRRTEITAN
3D structure
PDB6rku Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex.
ChainA
Resolution4.0 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A K42 V44 H78 H80 R272 K35 V37 H71 H73 R265
BS02 dna A I174 V176 K239 I167 V169 K232
BS03 JHN A D82 M120 D75 M113
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003916 DNA topoisomerase activity
GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008094 ATP-dependent activity, acting on DNA
GO:0034335 DNA negative supercoiling activity
GO:0042802 identical protein binding
Biological Process
GO:0006259 DNA metabolic process
GO:0006261 DNA-templated DNA replication
GO:0006265 DNA topological change
GO:0006351 DNA-templated transcription
GO:0009410 response to xenobiotic stimulus
GO:0046677 response to antibiotic
GO:0051276 chromosome organization
GO:2000104 negative regulation of DNA-templated DNA replication
Cellular Component
GO:0005694 chromosome
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0009330 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
GO:0016020 membrane

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:6rku, PDBe:6rku, PDBj:6rku
PDBsum6rku
PubMed31666516
UniProtP0AES4|GYRA_ECOLI DNA gyrase subunit A (Gene Name=gyrA)

[Back to BioLiP]