Structure of PDB 6p7e Chain A

Receptor sequence

>6p7eA (length=694) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSAIAANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGKRFGSHALEAWGYRLGEMKGEYKDDFKRMLEEQ
GEEYVDGMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTD
VGYTTFWSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARR
SELLRKLTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFKK
PPCELDTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKG
APVVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYV
AEDGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHH
LDGITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQ
IAAELPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTP
AIAALRESIQQTLVESSQWVAGEQQVKWKRRWIKGLDGRKVHVRSPHAAL
NTLLQSAGALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGC
RTEEIAQVVIETAQEAMRWVGDHWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

6p7e Combined Solution and Crystal Methods Reveal the Electrostatic Tethers That Provide a Flexible Platform for Replication Activities in the Bacteriophage T7 Replisome.

Chain

Resolution

3.001 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	K290 K293 V294 T357 K359 V363 V364 D365 D366 K394 R429 A438 Q439 I440 P441 G442 S445 H653	K290 K293 V294 T347 K349 V353 V354 D355 D356 K384 R419 A428 Q429 I430 P431 G432 S435 H643
BS02	dna	A	K299 K300 R318 S338 G402 K404 A425 H432 A433 N436 Q439 Y526 G527 Y530 A532 G533 K536 R604 H607 N611 Q615	K299 K300 R308 S328 G392 K394 A415 H422 A423 N426 Q429 Y516 G517 Y520 A522 G523 K526 R594 H597 N601 Q605
BS03	peptide	A	R490 R590 I593 K594 L613 R687	R480 R580 I583 K584 L603 R677
BS04	TTP	A	A7 A8 A10 Y64 W160	A7 A8 A10 Y64 W160
BS05	MG	A	D475 A476 D654	D465 A466 D644
BS06	MG	A	D475 D654 E655	D465 D644 E645
BS07	TTP	A	R429 D475 A476 G478 L479 E480 H506 R518 K522 Y526 D654	R419 D465 A466 G468 L469 E470 H496 R508 K512 Y516 D644

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

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Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:6p7e, PDBe:6p7e, PDBj:6p7e
PDBsum	6p7e
PubMed	31659895
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

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