Structure of PDB 6ozw Chain A

Receptor sequence

>6ozwA (length=538) Species: 210007 (Streptococcus mutans UA159)

PKKNLVIVESPAKAKTIEKYLGRSYKVVASVGHIRDLKKSSMSIDFDNNY
EPQYINIRGKGPLINSLKKEAKNAKQIFLASDPDREGEAISWHLAHILDL
DLKGKNRVVFNEITKDAVKNAFVEPRQIDMDLVDAQQARRVLDRIVGYSI
SPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFKPEEYWSIDGFFKKG
NKKFQANFYGLDNKKTKLKSNDDVKKVLTRIKNDDFLVDKVEKKERKRNA
PLPYTTSSLQQDAANKINFRTRKTMMVAQQLYEGIRLGSNGQQGLITYMR
TDSTRISPVAQNDAANYITEHFGAEYSKAHEAIRPSNVNHTPESIAKYLD
KDQLKLYTLIWNRFVASQMTAAVFDTVKVNLTQNGVLFIANGSQIKFKGY
MAVYTKVLPEMIKGETVKKISANPEQHFTQPPARYSEASLIKTLEENGVG
RPSTYAPTLETIQKRYYVRLVSKRFEPTELGEIVNSLIIEFFPDIVDVKF
TAEMESKLDEVEIGKEEWQKVIDQFYKPFEKEVIKAEE

3D structure

• PDB: 6ozw Crystal structure of the 65-kilodalton amino-terminal fragment of DNA topoisomerase I from the gram-positive model organism Streptococcus mutans.
• Chain: A
• Resolution: 2.063 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E27 S28 D100 D102 Y316 R318
• Catalytic site (residue number reindexed from 1): E9 S10 D82 D84 Y298 R300
• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	R158 R162	R140 R144
BS02	MG	A	I193 R196 E197	I175 R178 E179
BS03	MG	A	R157 D161	R139 D143

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003916 DNA topoisomerase activity
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

External links

• PDB: RCSB:6ozw, PDBe:6ozw, PDBj:6ozw
• PDBsum: 6ozw
• PubMed: 31204053
• UniProt: Q8DUD3