Structure of PDB 6osq Chain A

Receptor sequence

>6osqA (length=259) Species: 562 (Escherichia coli)

QVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRV
EIMSASEGEHGGYKEIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRI
HTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTTDSAIRI
THLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRN
LLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEPIIQ
EHQADQLAA

3D structure

• PDB: 6osq The structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy.
• Chain: A
• Resolution: 3.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R116 E161 F227 R228 S229 S230 A232 G233 G234 Q235 H236 V237 N238 T239 T240 D241 S242 R245 R261 Q263 H264 R277 R294 R295	R20 E65 F131 R132 S133 S134 A136 G137 G138 Q139 H140 V141 N142 T143 T144 D145 S146 R149 R165 Q167 H168 R181 R198 R199
BS02	rna	A	G118 T119 G120 G121 R133 R183 Q185 S192 Q193 R303 R306 Q314 R316	G22 T23 G24 G25 R37 R87 Q89 S96 Q97 R207 R210 Q218 R220
BS03	rna	A	G120 D122 E123 Q185 T190 E191 R195 I196 H197 T198	G24 D26 E27 Q89 T94 E95 R99 I100 H101 T102
BS04	rna	A	H156 G233 H236 R261	H60 G137 H140 R165

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0016149 translation release factor activity, codon specific

Biological Process

• GO:0006415 translational termination

External links

• PDB: RCSB:6osq, PDBe:6osq, PDBj:6osq
• PDBsum: 6osq
• PubMed: 31189921
• UniProt: P0A7I0|RF1_ECOLI Peptide chain release factor RF1 (Gene Name=prfA)