Structure of PDB 6or5 Chain A

Receptor sequence

>6or5A (length=2058) Species: 4896 (Schizosaccharomyces pombe)

DVLIEWVAIYPQIYDILEHINYVPSNTLQRLRLHQPWSKIDYDVWFLYAS
DEIRETCKVKYYGETKTYGEVFVLENERISQLHRLFVSERAEHLKNLLFD
ALPLVELGGNVFFNSHVPLPCSLVLTKSTQENLNRITPYLVQKRPILLAG
PEGIGKKFLITQIAAKLGQQIIRIHLSDSTDPKMLIGTYTWQPGVLTQAV
ITGWILFTNIEHEVLSVLLPLLEKRQLVIPSRGETIYAKGSFQMFATSSM
KTKILGQRLWQILDLTYQPDECVEVVSTLYPVLSIICPTLYSVYKDIFDL
FSSKIYRRLCLRDFYKFIKRVAFLYHKVFKEAIDMFGAFIPSRDGFDLVV
RNVAIELNIPPEKALQLPVFQNLEHNINIGRCSLKKLSTIRNSYAFTSSS
LGLLEQLAAGVQTNEPLLLVGETGTGKTTTIQLLAGLLGQKVTVINMSQQ
TESSDMLGSTLGLPLHERFIDIFEQTFSSKKNAKFISMASTSARRFRWKT
CLKIWKEACKLSKTVLNQVELRNQWAKFEKEVEDFEKVLTVEGALVKAVR
SGHWVLLDEINLASLETLEPIGQLLSPHKNFRLFGCMNPEPSFRSRFTEI
YVHSPDQNLDDLLSIIQKYIGSNEHVIREVAELYQVAKSLSLDPHYTVRT
LSRTLSYVTEIAPIYGLRRSLYEGFCMSFLTLLDHTSESLLYNHVVRFTL
NRDQQNAILKQIPKVPDYIAFCHYWLRRGPVEEQEHYIITPFVQKNLLNI
ARACSTRMPILIQGPTSSGKTSMIEYVAKKTGHKFVRINNHEHTDLQEYI
GTYVTDDNGSLSFREGVLVEALRNGYWIVLDELNLAPTDVLEALNRLLDD
NRELFIPQVLVKPHPEFMLFATQNPGRKHLSRAFRNRFLEIHFDDIPENE
LETILHKRCKIAPSYAAKIVQVFRELSLRRNSFATLRDLFRWAFREAVGY
QQLAENGYMLLAERARDQKDKLAVQEVIEKVMSKVVWTRPMIRLFCLVWR
CLLAKEPVLLVGDTGCGKTTVCQILAECLHKELHIINAHTENGDIIGAQR
PVRNRSAVNYSLHSQLCEKFNVIDDLIEKFEKLNLIERQIIKHDGALVTA
MKQFFLLDEISLADDSVLERLNSVLELSRTLTLAVSLTAKDGFAFFATMN
PGGKKELSPALRNRFTEIWVPTEDILKIVEGKLHNNKIELARPLVEYAKW
HANEYLYTDVISIRDVLSAVEFINACEILDLNLVLFNAVSMVFIDALLAS
LHAERQRCFAKLNELAHTDTTLMNASKVLRALQVLKPILLEGSPGVGKTS
LITALARETGHQLVRINLSDQTDLMDLFGSDVPVEAPFLAAMRNGHWVLL
DELNLASQSVLEGLNACLDHRNEAYIPELDKVFHPNFRVFAAQNPQGLPR
SFINRFSVVYVEALKEKDMIEIAACNYHQVNEDWRLKIIKFMFRLQDNIE
KDISGSPWEFNLRDTLRWLQLLNDAADYLEVMVLHRMRTVEDRVRTCELF
KEVFDIDYEPRTIGFSLSSQCFKVGHSLLVRDQKTLLDSQNILQSQLPVL
ESVITCINKKWPCILVGDTATGKTCILRLLAAIAGAKIKEMAVNSDTDTM
DLIDISRKASELFTDLSQQLLNIVIKYRNFDIFRETSLYTLTTSQAFTLL
QKVVDQLDETLVHSLGDIHEKARKLLAEFSASPAGGYLLKAVEEGHWFVL
DNANLCSPAVLDRLNSLLELIVNEKTTEDGHPKTIKPHPNFRLFLTVNPV
YGELSRAMRNRGVEIFLLKEALTEIDKKQMSLLEPAPISSAVDTLASNIS
YIKYVFETMGNYMYIAHAIILALFSPRQLKLLRKVLTLKNLVQKIYCADY
FNHMDLKASRFMDIYEYPVQLREVVGLIQTINDFQSVILTSHLELPETYA
SGLLFVSAHEILDLTEEVNTYLLKSASAVYGSTPSLWNLLNQFSKFLIEI
ASSNIVYKLDVIRHFLKLVVLWKNIYVWTSRFYCYTKMLGEWMFTKLLES
FLPKDSLEKFSELQTVESLRHYLESLYKESQILEIAESLNSGKVNSVMRR
LVSTEISN

3D structure

• PDB: 6or5 Structural Insights into Mdn1, an Essential AAA Protein Required for Ribosome Biogenesis.
• Chain: A
• Resolution: 4.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	A	L132 V133 G162 G164	L123 V124 G153 G155
BS02	ANP	A	S446 Y447 F449 T478 R777	S393 Y394 F396 T425 R649
BS03	ANP	A	I874 T903 G906 T908 S909	I738 T766 G769 T771 S772
BS04	ANP	A	V1167 T1195 G1196 C1197 G1198 K1199 T1201	V986 T1014 G1015 C1016 G1017 K1018 T1020
BS05	ANP	A	Q1849 N1850 L1852 T1878 A1879 G1881 C1884	Q1540 N1541 L1543 T1569 A1570 G1572 C1575

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0000055 ribosomal large subunit export from nucleus
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:6or5, PDBe:6or5, PDBj:6or5
• PDBsum: 6or5
• PubMed: 30318141
• UniProt: O94248|MDN1_SCHPO Midasin (Gene Name=mdn1)