Structure of PDB 6o0z Chain A

Receptor sequence

>6o0zA (length=1288) Species: 1314 (Streptococcus pyogenes)

KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDDVDKLFIQLVQTYNQLFEENPINAS
GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKS
NFDLAEDAKLQLSKDTYDDDLDNLLAQIDLFLAAKNLSDAILLSDILRVN
TEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGY
AGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS
IPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
KHSLLYEYFTVYNELTKVKYAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
YFKKIEASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMI
EERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRKTILDFLKSNR
NFMQLIHDDSLTFKEDIQKAQDSLHEHIANLAGSPAIKKGILQTVKVVDE
LVKVMGRHKPENIVIEMARRMKRIEEGIKELGSQILKEHPVENTQLQNEK
LYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQLKDDSIDNKVLTRSDK
NRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELD
KAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYKV
YDVRKMIYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDP
KKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNP
IDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELAL
PSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSK
RVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFD
TTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLG

3D structure

• PDB: 6o0z Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K30 L35 R40 K44 N46 E57 A59 T62 R63 K65 R66 R69 R70 R71 R74 K76 N77 S104 F105 R115 H116 I122 H129 F164 R165 G166 Y325 H328 H329 K336 R340 F351 Q402 R403 T404 F405 F446 R447 I448 L455 R457 N459 R467 T472 I473 H721 K742 P1090 V1100 G1103 G1104 F1105 I1110 P1112 R1122 K1124 A1227 Q1272 Q1350 S1351 Y1356	K28 L33 R38 K42 N44 E55 A57 T60 R61 K63 R64 R67 R68 R69 R72 K74 N75 S102 F103 R113 H114 I120 H127 F162 R163 G164 Y316 H319 H320 K327 R331 F342 Q393 R394 T395 F396 F437 R438 I439 L446 R448 N450 R458 T463 I464 H675 K696 P1012 V1022 G1025 G1026 F1027 I1032 P1034 R1044 K1046 A1149 Q1194 Q1272 S1273 Y1278
BS02	dna	A	Y450 W659 R661 K1107 E1108 S1109 K1334	Y441 W627 R629 K1029 E1030 S1031 K1256
BS03	dna	A	R1114 S1136 K1200 S1216 Q1221 R1335	R1036 S1058 K1122 S1138 Q1143 R1257

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6o0z, PDBe:6o0z, PDBj:6o0z
• PDBsum: 6o0z
• PubMed: 31285607
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)