Structure of PDB 6o0x Chain A

Receptor sequence

>6o0xA (length=1112) Species: 1314 (Streptococcus pyogenes)

KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
AAKAILSARLSKSRRLENLIAQLKKNGLFGNLIALSLGLTPNFKSNFDLA
EDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVN
TEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGY
AGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS
IPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKV
TVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDN
EENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGW
GRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQ
KAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEQI
TKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVRE
INNYHHAHDAYLNAVVGTALIKKRDFATVRKVLSMPQVNIVKKTEVQTGG
FSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKS
KKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ
KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITG
LYETRIDLSQLG

3D structure

• PDB: 6o0x Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.
• Chain: A
• Resolution: 3.28 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K30 L35 K44 N46 E57 T58 A59 T62 R63 R66 R69 R70 R71 R74 N77 L101 S104 F105 K111 R115 H116 I122 H129 K163 F164 R165 G166 H167 Y325 H328 H329 L332 K336 R340 F352 Y359 Q402 R403 T404 F405 F446 R447 I448 L455 R457 N459 S460 R467 I473 K510 Y515 N588 R661 K735 K742 P1090 G1103 G1104 F1105 I1110 P1112 R1122 K1124 A1227 Q1272 Q1350 S1351 Y1356	K28 L33 K42 N44 E55 T56 A57 T60 R61 R64 R67 R68 R69 R72 N75 L99 S102 F103 K109 R113 H114 I120 H127 K161 F162 R163 G164 H165 Y316 H319 H320 L323 K327 R331 F343 Y350 Q393 R394 T395 F396 F437 R438 I439 L446 R448 N450 S451 R458 I464 K501 Y506 N579 R652 K721 K728 P836 G849 G850 F851 I856 P858 R868 K870 A973 Q1018 Q1096 S1097 Y1102
BS02	dna	A	K1107 E1108 S1109	K853 E854 S855
BS03	dna	A	R1114 S1116 K1118 S1136 S1216 Q1221 R1333 R1335	R860 S862 K864 S882 S962 Q967 R1079 R1081
BS04	dna	A	Y450 N497 G582 T657 W659 M694 Q695 H698	Y441 N488 G573 T648 W650 M685 Q686 H689

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6o0x, PDBe:6o0x, PDBj:6o0x
• PDBsum: 6o0x
• PubMed: 31285607
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)