Structure of PDB 6nyb Chain A

Receptor sequence
>6nybA (length=350) Species: 9606 (Homo sapiens) [Search protein sequence]
TTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVR
DRSSSAPNVHDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVT
APTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLY
HHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDL
TVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSD
VYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKA
MKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGF
3D structure
PDB6nyb Architecture of autoinhibited and active BRAF-MEK1-14-3-3 complexes.
ChainA
Resolution4.1 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) D576 K578 N580 N581 D594 S616
Catalytic site (residue number reindexed from 1) D188 K190 N192 N193 D206 S228
Enzyme Commision number 2.7.11.1: non-specific serine/threonine protein kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 AGS A I463 G466 S467 F468 V471 A481 W531 C532 K578 N581 F583 D594 I75 G78 S79 F80 V83 A93 W143 C144 K190 N193 F195 D206
BS02 ZN A C248 C251 H269 C16 C19 H37
BS03 ZN A H235 T263 C264 C280 H3 T31 C32 C48
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004708 MAP kinase kinase activity
GO:0004709 MAP kinase kinase kinase activity
GO:0005509 calcium ion binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0031267 small GTPase binding
GO:0031434 mitogen-activated protein kinase kinase binding
GO:0042802 identical protein binding
GO:0044024 histone H2AS1 kinase activity
GO:0044877 protein-containing complex binding
GO:0046872 metal ion binding
GO:0097110 scaffold protein binding
GO:0106310 protein serine kinase activity
Biological Process
GO:0000165 MAPK cascade
GO:0001934 positive regulation of protein phosphorylation
GO:0002318 myeloid progenitor cell differentiation
GO:0006338 chromatin remodeling
GO:0006468 protein phosphorylation
GO:0007165 signal transduction
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008542 visual learning
GO:0009887 animal organ morphogenesis
GO:0010628 positive regulation of gene expression
GO:0010764 negative regulation of fibroblast migration
GO:0010828 positive regulation of D-glucose transmembrane transport
GO:0016079 synaptic vesicle exocytosis
GO:0016310 phosphorylation
GO:0030154 cell differentiation
GO:0030878 thyroid gland development
GO:0033077 T cell differentiation in thymus
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0034446 substrate adhesion-dependent cell spreading
GO:0035019 somatic stem cell population maintenance
GO:0036211 protein modification process
GO:0042127 regulation of cell population proliferation
GO:0043066 negative regulation of apoptotic process
GO:0043149 stress fiber assembly
GO:0043367 CD4-positive, alpha-beta T cell differentiation
GO:0043368 positive T cell selection
GO:0043369 CD4-positive or CD8-positive, alpha-beta T cell lineage commitment
GO:0043434 response to peptide hormone
GO:0043524 negative regulation of neuron apoptotic process
GO:0045580 regulation of T cell differentiation
GO:0046632 alpha-beta T cell differentiation
GO:0048538 thymus development
GO:0048679 regulation of axon regeneration
GO:0048680 positive regulation of axon regeneration
GO:0050772 positive regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0051496 positive regulation of stress fiber assembly
GO:0051591 response to cAMP
GO:0060291 long-term synaptic potentiation
GO:0060323 head morphogenesis
GO:0060324 face development
GO:0070371 ERK1 and ERK2 cascade
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071277 cellular response to calcium ion
GO:0071466 cellular response to xenobiotic stimulus
GO:0072577 endothelial cell apoptotic process
GO:0090150 establishment of protein localization to membrane
GO:0099170 postsynaptic modulation of chemical synaptic transmission
GO:1900026 positive regulation of substrate adhesion-dependent cell spreading
GO:1902531 regulation of intracellular signal transduction
GO:1990090 cellular response to nerve growth factor stimulus
GO:2000301 negative regulation of synaptic vesicle exocytosis
GO:2000352 negative regulation of endothelial cell apoptotic process
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0043005 neuron projection
GO:0043231 intracellular membrane-bounded organelle
GO:0044297 cell body
GO:0098793 presynapse
GO:0098794 postsynapse
GO:0098978 glutamatergic synapse

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:6nyb, PDBe:6nyb, PDBj:6nyb
PDBsum6nyb
PubMed31581174
UniProtP15056|BRAF_HUMAN Serine/threonine-protein kinase B-raf (Gene Name=BRAF)

[Back to BioLiP]