Structure of PDB 6nus Chain A

Receptor sequence

>6nusA (length=715)

RLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVE
NPDILRVYANLGERVRQSLLKTVQFCDAMRDAGIVGVLTLDNQDLNGNWY
FVQVAPGCGVPIVDSYYSLLMPILTLTRALAAESHMDADLAKPLIKWDLL
KYDFTEERLCLFDRYFKYWDQTYHPNCINCLDDRCILHCANFNVLFSTVF
PPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKEL
LVYAADPAMHAASGNLLLDKRTTCFSVAALTNGNFNKDFYDFAVSKYRYN
LPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKW
GKARLYYDSMSYEDQDALFAYTKRNVIPTITQMVSICSTMTNRQFHQKLL
KSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVETPHLMGWDYPKCDRAM
PNMLRIMASLVLARKHNTCCNLSHRFYRLANECAQVLSEMVMCGGSLYVK
PGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHR
LYECLYRNRDVDHEFVDEFYAYLRKHFSMMILSDDAVVCYNSNYAAQGLV
ASIKNFKAVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDY
VYLPYPDPSRILGALMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIR
KLMLTNDNTSRYWEP

3D structure

• PDB: 6nus Structure of the SARS-CoV nsp12 polymerase bound to nsp7 and nsp8 co-factors.
• Chain: A
• Resolution: 3.5 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.-
  2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	H295 C301 C306 C310	H174 C180 C185 C189
BS02	ZN	A	H642 C645 C646	H466 C469 C470

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:6nus, PDBe:6nus, PDBj:6nus
• PDBsum: 6nus
• PubMed: 31138817
• UniProt: P0C6X7|R1AB_SARS Replicase polyprotein 1ab (Gene Name=rep)