Structure of PDB 6nef Chain A

Receptor sequence

>6nefA (length=407) Species: 243231 (Geobacter sulfurreducens PCA)

FHSGGVAECEGCHTMHNSLGGAVMNSATAQFTTGPMLLQGATQSSSCLNC
HQHAGDTGPSSYHISTAEADMPAGTAPLQMTPGGDFGWVKKTYTWNVRGL
NTSEGERKGHNIVAGDYNYVADTTLTTAPGGTYPANQLHCSSCHDPHGKY
RRFVDGSIATTGLPIKNSGSYQNSNDPTAWGAVGAYRILGGTGYQPKSLS
GSYAFANQVPAAVAPSTYNRTEATTQTRVAYGQGMSEWCANCHTDIHNSA
YPTNLRHPAGNGAKFGATIAGLYNSYKKSGDLTGTQASAYLSLAPFEEGT
ADYTVLKGHAKIDDTALTGADATSNVNCLSCHRAHASGFDSMTRFNLAYE
FTTIADASGNSIYGTDPNTSSLQGRSVNEMTAAYYGRTADKFAPYQRALC
NKCHAKD

3D structure

• PDB: 6nef Cryo-EM reveals the structural basis of long-range electron transport in a cytochrome-based bacterial nanowire.
• Chain: A
• Resolution: 3.4 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	A	V31 C34 C37 H38 M61 L63 H172 P221 K222	V6 C9 C12 H13 M36 L38 H147 P196 K197
BS02	HEC	A	F26 H27 C72 C75 H76 Y87 H169 S195 R212	F1 H2 C47 C50 H51 Y62 H144 S170 R187
BS03	HEC	A	I89 T106 P107 G108 C165 C168 H169 Y211 R212 H360	I64 T81 P82 G83 C140 C143 H144 Y186 R187 H335
BS04	HEC	A	R132 H135 N136 D147 L150 A160 C264 C267 H268 H357 M367	R107 H110 N111 D122 L125 A135 C239 C242 H243 H332 M342
BS05	HEC	A	P154 S261 H268 I271 C353 C356 H357	P129 S236 H243 I246 C328 C331 H332
BS06	HEC	A	Y276 H282 P283 F290 C425 C428 H429	Y251 H257 P258 F265 C400 C403 H404
BS07	MG	A	E131 N136 D147	E106 N111 D122

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0046872 metal ion binding

Biological Process

• GO:0009061 anaerobic respiration

Cellular Component

• GO:0009279 cell outer membrane
• GO:0009986 cell surface

External links

• PDB: RCSB:6nef, PDBe:6nef, PDBj:6nef
• PDBsum: 6nef
• PubMed: 31240257
• UniProt: Q74A86|OMCS_GEOSL C-type cytochrome OmcS (Gene Name=omcS)