Structure of PDB 6mt8 Chain A

Receptor sequence

>6mt8A (length=159) Species: 83333 (Escherichia coli K-12)

MISLIAALAVDRVIGMMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRP
LPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQF
LPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCF
EILERRHHH

3D structure

• PDB: 6mt8 Time-resolved x-ray crystallography capture of a slow reaction tetrahydrofolate intermediate.
• Chain: A
• Resolution: 1.35 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): I5 M20 W22 D27 L28 F31 L54 I91 T113
• Catalytic site (residue number reindexed from 1): I5 M17 W19 D24 L25 F28 L51 I88 T110
• Enzyme Commision number: 1.5.1.3: dihydrofolate reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	THG	A	A6 A7 D27 L28 F31 K32 I50 R57 Y100	A6 A7 D24 L25 F28 K29 I47 R54 Y97
BS02	DHF	A	A6 A7 D27 F31 I50 L54 R57 I94	A6 A7 D24 F28 I47 L51 R54 I91

Gene Ontology

Molecular Function

• GO:0004146 dihydrofolate reductase activity
• GO:0005515 protein binding
• GO:0005542 folic acid binding
• GO:0016491 oxidoreductase activity
• GO:0050661 NADP binding
• GO:0051870 methotrexate binding
• GO:0051871 dihydrofolic acid binding
• GO:0070401 NADP+ binding
• GO:0070402 NADPH binding

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0009257 10-formyltetrahydrofolate biosynthetic process
• GO:0009410 response to xenobiotic stimulus
• GO:0031427 response to methotrexate
• GO:0046452 dihydrofolate metabolic process
• GO:0046654 tetrahydrofolate biosynthetic process
• GO:0046655 folic acid metabolic process
• GO:0046656 folic acid biosynthetic process
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:6mt8, PDBe:6mt8, PDBj:6mt8
• PDBsum: 6mt8
• PubMed: 30868089
• UniProt: P0ABQ4|DYR_ECOLI Dihydrofolate reductase (Gene Name=folA)