Structure of PDB 6mn8 Chain A

Receptor sequence

>6mn8A (length=399) Species: 6282 (Onchocerca volvulus)

AHHHHHHIEISKDENYSEWYVQVITKAEMIEYYDISGCYVLRPWSYAIWE
FIQEWFDEEIKKLGVKNCYFPLFVSQSALEKEFAPEVAWITRAGQSDLAE
AIAIRPTSETVMYPSYAKWVQSHRDLPIKLNQWCNVVRWEFKHPTPFLRT
REFLWQEGHTAFQSKDEAEDEVFKILDLYAQIYIDLLAIPVIKGRKGGDF
TATVEAYVPVNGRGIQGATSHHLGQNFSKMFNISFEDPNGGGKIYAWQNS
WGISTRTIGALVMIHGDNCGLVLPPRVATIQMIIVPVGITKDEQKTALIE
KAKEINNKLMDASIRAELDIRDHISPGWKFNHWELKGVPVRIEIGPKDLA
NNQVTCVIRYSGEKRTIPIDGLASKCKDMLEEIHYSMYNRILEVRESHT

3D structure

• PDB: 6mn8 Crystal Structure of Prolyl-tRNA Synthetase from Onchocerca volvulus with bound Halofuginone and nucleotide
• Chain: A
• Resolution: 2.35 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.15: proline--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	V172 S293	V120 S234
BS02	HFG	A	F135 E138 P158 T159 E161 R190 H280 S309 W310 G311	F83 E86 P106 T107 E109 R138 H221 S250 W251 G252
BS03	ANP	A	R190 E192 R201 T202 F205 W207 Q275 A277 T278 S313 R315	R138 E140 R149 T150 F153 W155 Q216 A218 T219 S254 R256
BS04	AMP	A	R190 E192 T202 F205 Q275 A277 T278 S313 R315	R138 E140 T150 F153 Q216 A218 T219 S254 R256
BS05	2PN	A	R201 R315	R149 R256
BS06	SER	A	W101 L238	W55 L186

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004827 proline-tRNA ligase activity
• GO:0005524 ATP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006433 prolyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0017101 aminoacyl-tRNA synthetase multienzyme complex

External links

• PDB: RCSB:6mn8, PDBe:6mn8, PDBj:6mn8
• PDBsum: 6mn8
• UniProt: A0A2K6VKP7