Structure of PDB 6mfz Chain A

Receptor sequence

>6mfzA (length=1789) Species: 54914 (Brevibacillus parabrevis)

MGRILFLTTFMSKGNKVVRYLESLHHEVVICQEKVHAQSANLQEIDWIVS
YAYGYILDKEIVSRFRGRIINLHPSLLPWNKGRDPVFWSVWDETPKGVTI
HLIDEHVDTGDILVQEEIAFADEDTLLDCYNKANQAIEELFIREWENIVH
GRIAPYRQTAGGTLHFKADRDFYKNLNMTTVRELLALKRLCAEPKRGEKP
IDKTFHQLFEQQVEMTPDHVAVVDRGQSLTYKQLNERANQLAHHLRGKGV
KPDDQVAIMLDKSLDMIVSILAVMKAGGAYVPIDPDYPGERIAYMLADSS
AAILLTNALHEEKANGACDIIDVHDPDSYSENTNNLPHVNRPDDLVYVMY
TSGSTGLAKGVMIEHHNLVNFCEWYRPYFGVTPADKALVYSSFSFDGSAL
DIFTHLLAGAALHIVPSERKYDLDALNDYCNQEGITISYLPTGAAEQFMQ
MDNQSFRVVITGGDVLKKIERNGTYKLYNGYGPTECTIMVTMFEVDKPYA
NIPIGKPIDRTRILILDEALALQPIGVAGELFIVGEGLGRGYLNRPELTA
EKFIVHPQTGERMYRTGDRARFLPDGNIEFLGRLDNLVKIRGYRIEPGEI
EPFLMNHPLIELTTVLAKEQADGRKYLVGYYVAPEEIPHGELREWLGNDL
PDYMIPTYFVHMKAFPLTANGKVDRRALPDVQADVAPTDELEQQLAQVWS
HVLGIPQMGIDDHFLERGGDSIKVMQLIHQLKNIGLSLRYDQLFTHPTIR
QLKRLLTEQKQVSLEPLRELDEQAEYETSAVEKRMYIIQQQDVESIAYNV
VYTINFPLTVDTEQIRVALEQLVLRHEGLRSTYHMRGDEIVKRIVPRAEL
SFVRQTGEEESVQSLLAEQIKPFDLAKAPLLRAGVIETADKKVLWFDSHH
ILLDGLSKSILARELQALLGQQVLSPVEKTYKSFARWQNEWFASDEYEQQ
IAYWKTLLQGELPAVQLPTKKRPPQLTFDGAIQMYRVNPEITRKLKATAA
KHDLTLYMLMLTIVSIWLSKMNSDSNQVILGTVTDGRQHPDTRELLGMFV
NTLPLLLSIDHEESFLHNLQQVKAKLLPALQNQYVPFDKILEAARVKREG
NRHPLFDVMFMMQGAPETELESNMHHINAGISKFDLTLEVLERENGLNIV
FEYNTHLFDEGMILRMVAQFEHLLLQAVHGLDQQVKRFELVTEDEKRDLF
LRVNDTAKAYPNKLIMSMLEDWAAATPDKTALVFREQRVTYRELNERVNQ
LAHTLREKGVQPDDLVMLMAERSVEMMVAIFAVLKAGGAYLPIDPHSPAE
RIAYIFADSGAKLVLAQSPFVEKASMAEVVLDLNSASSYAADTSNPPLVN
QPGDLVYVMYTSGSTGKPKGVMIEHGALLNVLHGMQDEYPLLQDDAFLLK
TTYIFDISVAEIFGWVPGRGKLVILEPEAEKNPKAIWQAVVGAGITHINF
VPSMLIPFVEYLEGRTEANRLRYILACGEAMPDELVPKVYEVLPEVKLEN
IYGPTEATIYASRYSLAKGSQESPVPIGKPLPNYRMYIINRHGQLQPIGV
PGELCIAGASLARGYLNNPALTEEKFTPHPLEKGERIYRTGDLARYREDG
NIEYLGRMDHQVKIRGYRIELDEIRSKLIQEETIQDAVVVARNDQNGQAY
LCAYLLSEQEWTVGQLRELLRRELPEYMIPAHFVLLKQFPLTANGKLDRK
ALPEAAPRTELEATLAHIWGEVLGIERIGIRDNFFELGGDSIKGLQIASR
LQRINWTMVINHLFLYPTIEQIAPFVTSEQVVIEAAAEN

3D structure

• PDB: 6mfz Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility.
• Chain: A
• Resolution: 6.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T351 F371 T484 E485 K589 R594 K672
• Catalytic site (residue number reindexed from 1): T351 F371 T484 E485 K589 R594 K672

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PNS	A	R1106 S1760	R1098 S1741
BS02	PNS	A	S729 Y748 Y1015	S721 Y740 Y1007

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0031177 phosphopantetheine binding

Biological Process

• GO:0009058 biosynthetic process

External links

• PDB: RCSB:6mfz, PDBe:6mfz, PDBj:6mfz
• PDBsum: 6mfz
• PubMed: 31699907
• UniProt: Q70LM7|LGRA_BREPA Linear gramicidin synthase subunit A (Gene Name=lgrA)