Structure of PDB 6mfy Chain A

Receptor sequence

>6mfyA (length=1598) Species: 54914 (Brevibacillus parabrevis)

MGRILFLTTFMSKGNKVVRYLESLHHEVVICQEKVHAQSANLQEIDWIVS
YAYGYILDKEIVSRFRGRIINLHPSLLPWNKGRDPVFWSVWDETPKGVTI
HLIDEHVDTGDILVQEEIAFADEDTLLDCYNKANQAIEELFIREWENIVH
GRIAPYRQTAGGTLHFKADRDFYKNLNMTTVRELLALKRLCAKPIDKTFH
QLFEQQVEMTPDHVAVVDRGQSLTYKQLNERANQLAHHLRGKGVKPDDQV
AIMLDKSLDMIVSILAVMKAGGAYVPIDPDYPGERIAYMLADSSAAILLT
NALHEEKANGACDIIDVHDPDSYSENTNNLPHVNRPDDLVYVMYTSGSTG
LAKGVMIEHHNLVNFCEWYRPYFGVTPADKALVYSSFSFDGSALDIFTHL
LAGAALHIVPSERKYDLDALNDYCNQEGITISYLPTGAAEQFMQMDNQSF
RVVITGGDVLKKIERNGTYKLYNGYGPTECTIMVTMFEVDKPYANIPIGK
PIDRTRILILDEALALQPIGVAGELFIVGEGLGRGYLNRPELTAEKFIVH
PQTGERMYRTGDRARFLPDGNIEFLGRLDNLVKIRGYRIEPGEIEPFLMN
HPLIELTTVLAKEQADGRKYLVGYYVAPEEIPHGELREWLGNDLPDYMIP
TYFVHMKAFPLTANGKVDRRALPDVLLGEDYVAPTDELEQQLAQVWSHVL
GIPQMGIDDHFLERGGDSIKVMQLIHQLKNIGLSLRYDQLFTHPTIRQLK
RLLTEQKLEPLRELDEQAEYETSAVEKRMYIIQQQDVESIAYNVVYTINF
PLTVDTEQIRVALEQLVLRHEGLRSTYHMRGDEIVKRIVPRAELSFVRQT
GEEESVQSLLAEQIKPFDLAKAPLLRAGVIETADKKVLWFDSHHILLDGL
SKSILARELQALLGQQVLSPVEKTYKSFARWQNEWFASDEYEQQIAYWKT
LLQGELPAVQLPTKKRPPQLTFDGAIQMYRVNPEITRKLKATAAKHDLTL
YMLMLTIVSIWLSKMNSDSNQVILGTVTDGRQHPDTRELLGMFVNTLPLL
LSIDHEESFLHNLQQVKAKLLPALQNQYVPFDKILEAARVKREGNRHPLF
DVMFMMQGAPETELESNMHHINAGISKFDLTLEVLERENGLNIVFEYNTH
LFDEGMILRMVAQFEHLLLQAVHGLDQQVKRFELVTEDEKRDLFLRVNDT
AKAYPNKLIMSMLEDWAAATPDKTALVFREQRVTYRELNERVNQLAHTLR
EKGVQPDDLVMLMAERSVEMMVAIFAVLKAGGAYLPIDPHSPAERIAYIF
ADSGAKLVLAQSPFVEKASMAEVVLDLNSASSYAADTSNPPLVNQPGDLV
YVMYTKPKGVMIEHGALLNVLHGMQDEYPLLQDDAFLLKTTYIFDISVAE
IFGWVPGRGKLVILEPEAEKNPKAIWQAVVGAGITHINFVPSMLIPFVEY
LEGRTEANRLRYILACGEAMPDELVPKVYEVLPEVKLENIYGPTEATIYA
SRYSLAKGSQESPVPIGKPLPNYRMYIINRHGQLQPIGVPGELCIAGASL
ARGYLNNPALTEEKFTPHPLEKGERIYRTGDLARYREDGNIEYLGRMD

3D structure

• PDB: 6mfy Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T351 F371 T484 E485 K589 R594 K672
• Catalytic site (residue number reindexed from 1): T345 F365 T478 E479 K583 R588 K666

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PNS	A	S729 H908 G913 T1013 Y1015 V1041 T1042 D1043	S718 H894 G899 T999 Y1001 V1027 T1028 D1029

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity

Biological Process

• GO:0009058 biosynthetic process

External links

• PDB: RCSB:6mfy, PDBe:6mfy, PDBj:6mfy
• PDBsum: 6mfy
• PubMed: 31699907
• UniProt: Q70LM7|LGRA_BREPA Linear gramicidin synthase subunit A (Gene Name=lgrA)